梁建娣;赵良功;刘小花;李文;党子龙;梁瑾;封士兰;
LIANG Jian-di1,ZHAO Liang-gong2,LIU Xiao-hua1,LI Wen1,DANG Zi-long1,LIANG Jin1,FENG Shi-lan1(1.School of Pharmaceutical,Lanzhou University,Lanzhou 730000,China; 2.The Second Clinical Medical College of Lanzhou University,Lanzhou 730000,China)

• 1:兰州大学药学院
• 2:兰州大学第二临床医学院

摘要(Abstract)：

目的:分析少毛北前胡氯仿提取部位高效液相色谱法(HPLC)指纹图谱与化痰药效关联性,以确立其化痰"药效成分组"。方法:通过HPLC测定分析少毛北前胡氯仿提取部位HPLC指纹图谱,用小鼠酚红化痰法研究其化痰作用,急支糖浆为阳性对照药(12 mL.kg-1),给小鼠灌胃氯仿提取物6 d(1.4 g.kg-1),用分光光度计测定小鼠气管的酚红分泌量,然后用灰关联度分析方法研究其谱效关系。结果:少毛北前胡氯仿提取部位的化痰作用是其所有化学成分共同作用的结果,各特征峰代表各自化学成分,其对化痰作用贡献的大小顺序(按特征峰编号)为13>12>16>18>19>6>20>14>1>11>15>10>17>2>5>4>7>3>8>9,其中1,3,4,10,13,16号特征峰鉴定为异紫花前胡苷元,补骨脂素,花椒毒素,Pd-Ib,蝉翼素,peuformosin。结论:少毛北前胡氯仿提取部位具有较强化痰活性,其HPLC指纹图谱与化痰作用之间有一定对应关系。
Objective:To analyze the relationship between high-performance liquid chromatography(HPLC) fingerprints of the chloroform extract fractions of Peucedanum harrysmithii var.subglabrum(PHS) and its phlegm-reducing effect,in order to establish″active component group for reducing phlegm″.Method:HPLC was adopted to determine and analyze HPLC fingerprints of chloroform extract fractions of PHS.Phenol red expectorant experiment was used to observe the phlegm-reducing effect in mice.Mice were administered intragastrically with chloroform extract fractions for 6 days(1.4 g·kg-1),with acute bronchitis syrup as the positive control drug(12 mL·kg-1).The phenol red secretion in mice was determined by spectrophotometer.Then the grey relational analysis was used to study the spectrum-effect relationship.Result:The phlegm-reducing effect of the chloroform extract fractions of PHS were resulted from the combined effect of all of its chemical components.Its various characteristic peaks represented different chemical components,and the order of their contributions to the phlegm-reducing effect was(number of peaks) 13>12>16>18>19>6>20>14>1>11>15>10>17>2>5>4>7>3>8>9,in No.1,3,4,10,13 and 16 characteristic peaks were identified as marmesin,psoralen,xanthotoxin,Pd-Ib,pteryxin and peuformosin.Conclusion:The chloroform extract fractions of PHS show strongly phlegm-reducing effect.There may be certain relationship between their HPLC fingerprint and phlegm-reducing effect.

关键词(KeyWords)： 少毛北前胡;指纹图谱;化痰作用;灰关联度;谱效关系
Peucedanum harrysmithii var.subglabrum;fingerprint;phlegm-reducing effect;grey relational analysis;spectrum-effect relationship

Abstract:

Keywords:

基金项目(Foundation): 甘肃省科技计划项目(1011WCGA162);; 兰州大学中央高校基本科研业务费专项资金项目(lzujbky-2010-191);; 教育部“春晖计划”合作科研项目(Z2009-1-62002);; 兰州大学国家大学生创新性实验计划项目(101073039);; 兰州市科技计划项目(2011-1-133)

作者(Author): 梁建娣;赵良功;刘小花;李文;党子龙;梁瑾;封士兰;
LIANG Jian-di1,ZHAO Liang-gong2,LIU Xiao-hua1,LI Wen1,DANG Zi-long1,LIANG Jin1,FENG Shi-lan1(1.School of Pharmaceutical,Lanzhou University,Lanzhou 730000,China; 2.The Second Clinical Medical College of Lanzhou University,Lanzhou 730000,China)

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DOI:

参考文献(References)：

• [1]中国药典.一部[S].2010:248.
• [2]陈二林,刘小花,李文,等.高效液相色谱法同时测定少毛北前胡中6种香豆素[J].分析化学研究简报,2009,37(8):1227.
• [3]张丽杰,刘丽娟,齐凤琴,等.中药谱效关系研究进展[J].中国现代应用药学,2010,27(11):971.
• [4]顾英,冯怡,李玉敏.指纹图谱在中药物质基础研究中的应用[J].中成药,2007,29(7):1048.
• [5]李文,封士兰,胡芳弟,等.毛北前胡的香豆素类化学成分研究[J].中国中药杂志,2009,34(10):1231.
• [6]张村,殷小杰,李丽,等.白花前胡蜜炙前后的药效学比较研究[J].中国实验方剂学杂志,2010,16(15):146.
• [7]孙芳芳.浅议灰色关联度分析方法及其应用[J].科技信息,2010(17):364.
• [8]邓聚龙.灰色系统理论教程[M].武汉:华中理工大学出版社,1990.