中国中药杂志

2019, v.44(24) 5363-5367

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青蒿新品种“科蒿1号”的选育研究
Breeding of new Artemisia annua variety "Kehao No.1"

梁妍;周翔;郭建钊;张梅;江红格;付晨青;付运星;石紫薇;刘宇;辛志君;陆锡宏;梁剑平;郝宝成;李雪虎;王震;
LIANG Yan;ZHOU Xiang;GUO Jian-zao;ZHANG Mei;JIANG Hong-ge;FU Chen-qing;FU Yun-xing;SHI Zi-wei;LIU Yu;XIN Zhi-jun;LU Xi-hong;LIANG Jian-ping;HAO Bao-cheng;LI Xue-hu;WANG Zhen;School of Pharmacy,Lanzhou University;Institute of Modern Physics,Chinese Academy of Sciences;Henan Yuzhou Tianyuan Biotechnology Co.,Ltd.;Jiangsu Siweisen Bioengineering Technology Center Co.,Ltd.;Lanzhou Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences;

摘要(Abstract):

青蒿是抗疟疾一线药物青蒿素的天然植物来源,其青蒿素含量的高低直接影响着后续青蒿素的提取工艺和青蒿素的提取量。目前常常使用传统育种方法结合组织培养等进行选育高产青蒿素的青蒿新品种,但该选育方法具有效率低,需要持续选育等缺点。该研究利用重离子束辐照技术以青蒿栽培群体特异性种质资源为材料,以形态特征、农艺性状和青蒿素含量为指标,观察历代选育材料,进行品比试验和区域试验,培育新品种,并对该品种进行了SRAP分子标记技术鉴定。结果发现,选育的青蒿新品种"科蒿1号",平均干叶亩产量235. 0 kg,较对照增产20%以上;平均青蒿素质量分数为2. 0%,比对照提高45%,高抗白粉病,为高产优质新品种。重离子束辐照诱变育种可明显提高青蒿产量和青蒿素含量,"科蒿1号"具有良好的推广价值。
As a natural plant source of artemisinin,a first-line drug against malaria,Artemisia annua directly affects the extraction process of artemisinin and the source of artemisinin. At present,traditional breeding methods combined with tissue culture are often used to breed high-yield artemisinin-containing new varieties of A. annua. However,the breeding method has the disadvantages of low efficiency and continuous selection. In this study,heavy ion beam irradiation technology was used to observe the specific germplasm resources of A. annua,and the morphological characteristics,agronomic traits and artemisinin content were used as indicators to observe the selection materials and materials. The cultivated new varieties were compared with trials and regional trials. In addition,the new variety of A. annua was identified by SRAP molecular marker technology. The results showed that the new variety of A. annua, " Kehao No.1",had an average yield of 235. 0 kg of dry leaf per mu,which was more than 20% higher than that of the control. Especially,the average artemisinin content was 2. 0%,which was 45% higher than that of the control,and the " Kehao No.1" has high anti-white powder disease,high-yield and high-quality new varieties. Therefore,mutagenic breeding of heavy ion beam irradiation can significantly improve the yield and artemisinin content of the " Kehao No. 1" and it has a good promotion value.

关键词(KeyWords): 青蒿(黄花蒿);重离子束;新品种;青蒿素
Artemisia annua;heavy ion beam;new variety;artemisinin

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2017ZX09101002-003-001);; 甘肃省中药材产业体系项目

作者(Author): 梁妍;周翔;郭建钊;张梅;江红格;付晨青;付运星;石紫薇;刘宇;辛志君;陆锡宏;梁剑平;郝宝成;李雪虎;王震;
LIANG Yan;ZHOU Xiang;GUO Jian-zao;ZHANG Mei;JIANG Hong-ge;FU Chen-qing;FU Yun-xing;SHI Zi-wei;LIU Yu;XIN Zhi-jun;LU Xi-hong;LIANG Jian-ping;HAO Bao-cheng;LI Xue-hu;WANG Zhen;School of Pharmacy,Lanzhou University;Institute of Modern Physics,Chinese Academy of Sciences;Henan Yuzhou Tianyuan Biotechnology Co.,Ltd.;Jiangsu Siweisen Bioengineering Technology Center Co.,Ltd.;Lanzhou Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences;

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