中国中药杂志

2011, v.36(09) 1202-1206

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蔓性千斤拔药材的HPLC特征指纹图谱
HPLC characteristic fingerprint of Moghania philippinensis root

蒙蒙;闫利华;宋小妹;张启伟;王智民;
MENG Meng1,2,3,YAN Lihua1,3,SONG Xiaomei2,ZHANG Qiwei1,3,WANG Zhimin1,3(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Shanxi University of Chinese Medicine,Xianyan 712046,China;3.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Beijing 100700,China)

摘要(Abstract):

目的:研究蔓性千斤拔药材的HPLC指纹图谱,为科学评价、有效控制药材质量提供可靠方法。方法:采用HPLC色谱法,Kromasil C18色谱柱(4.6 mm×250 mm,5μm),0.3%乙酸水-乙腈洗脱系统,梯度洗脱,检测波长254 nm,柱温40℃。结果:建立了蔓性千斤拔药材HPLC指纹图谱分析方法;对23批样品进行了分析,其中21批样品相似度达到0.80以上;确立了22个共有峰,并指认了5个峰,分别为染料木素、染料木苷、5,7,3′,4′-四羟基-6,8-双异戊烯基异黄酮、5,7,4′-三羟基-6,8-双异戊烯基异黄酮、flemiphilippinin E。结论:该方法简便、准确、重现性好,可用于蔓性千斤拔指纹图谱的测定,并为评价药材的质量提供依据。
Objective:To study HPLC characteristic fingerprint of Moghania philippinensis and provide a reliable method for scientific evaluation and quality control.Method: The chromatographic separation was performed on a Kromasil C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile and water containing 0.3% acetic acid in gradient elution.The column temperature was set at 40℃ and the detection wavelength was at 254 nm.Result: The developed HPLC fingerprint method above was applied to analyze 23 batches of crude herbs of Moghania philippinensis.The total 22 peaks were selected as the characteristic peaks.And five of them were identified as genistein,genistin,5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone,5,7,4′-trihydroxy-6,8-diprenylisoflavone and flemiphilippinin E by matching their retention time,UV spectra data with those of the authentic chemical reference substances.Conclusion: The method is simple,accurate and reproducible.It is suitable for the quality control of M.philippinensis.

关键词(KeyWords): 蔓性千斤拔;特征指纹图谱;HPLC;异黄酮
Moghania philippinensis;characteristic fingerprint;HPLC;isoflavone

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003、2009ZX09301-005);; 中国中医科学院自主选题项目(Z02072)

作者(Author): 蒙蒙;闫利华;宋小妹;张启伟;王智民;
MENG Meng1,2,3,YAN Lihua1,3,SONG Xiaomei2,ZHANG Qiwei1,3,WANG Zhimin1,3(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Shanxi University of Chinese Medicine,Xianyan 712046,China;3.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Beijing 100700,China)

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