中国中药杂志

2013, v.38(05) 757-761

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常用藏药“蒂达(藏茵陈)”各品种的薄层色谱鉴别研究
Study on TLC identification of Dida commonly used in Tibetan medicine from different species

阳勇;钟国跃;武小赟;罗维早;谷丹丹;
YANG Yong1,ZHONG Guo-yue1,2,WU Xiao-yun1,3,LUO Wei-zao1*,GU Dan-dan1,3(1.Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China; 2.Jiangxi University of Traditional Chinese Medicine,Jiangxi 330004,China; 3.Chengdu University of Traditional Chinese Medicine,Chengdu 611730,China)

摘要(Abstract):

目的:建立藏药"蒂达"不同药材品种的薄层色谱鉴别方法。方法:采用硅胶G板、三氯甲烷-甲醇(40∶1)展开,分离鉴别"蒂达"不同药材品种中的齐墩果酸成分。结果:对70个来源于龙胆科獐牙菜属、花锚属、扁蕾属、肋柱花属及虎耳草科虎耳草属的"蒂达"各品种药材中齐墩果酸的薄层色谱鉴别表明,除虎耳草外,其他来源品种中均检出有齐墩果酸,同种的各样品间薄层图谱特征一致,但同属不同种间及同科不同属间薄层图谱特征存在一定差异。结论:齐墩果酸的薄层鉴别方法可用于"藏茵陈"药材部分种的鉴别,可以有效鉴别来源于虎耳草科与其他种的"蒂达"样品。
Objective: To establish a method of TLC identification for Dida commonly used in Tibetan medicine from different species.Method: With silica gel G as the stationary phase,and chloroform-methanol(40∶1) as mobile phase,oleanolic acid from different species of Dida was separated and identified.Result: Oleanolic acid was detected in 70 kinds of Dida derived from the Gentianaceae Swertia,Halenia,Gentianopsis,Lomatogonium,and Saxifragaceae saxifrage,except for the saxifrage,there are some differences among different genera or subjection.Conclusion: This TLC method can be used for identification of oleanolic acid in Dida from different species except saxifrage.

关键词(KeyWords): 藏茵陈;薄层色谱鉴别;齐墩果酸
Dida;TLC;oleanolic acid

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基金项目(Foundation): 国家科技支撑计划项目子课题(2006DA106A11-12);; 创新药物孵化基地项目子课题(2010ZX09401-306-3-9);; 国家科技重大专项子课题(2012ZX09103201-050)

作者(Author): 阳勇;钟国跃;武小赟;罗维早;谷丹丹;
YANG Yong1,ZHONG Guo-yue1,2,WU Xiao-yun1,3,LUO Wei-zao1*,GU Dan-dan1,3(1.Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China; 2.Jiangxi University of Traditional Chinese Medicine,Jiangxi 330004,China; 3.Chengdu University of Traditional Chinese Medicine,Chengdu 611730,China)

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