中国中药杂志

2022, v.47(10) 2681-2688

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基于植物代谢组学和指标成分的黄芩药材优质标准研究
High quality standard of Scutellariae Radix based on plant metabolomics and index components

张娜;苏风山;周园涛;高启国;陈军力;徐晓丽;韩玲玉;
ZHANG Na;SU Feng-shan;ZHOU Yuan-tao;GAO Qi-guo;CHEN Jun-li;XU Xiao-li;HAN Ling-yu;Traditional Chinese Medicine Institute, School of Pharmaceutical Sciences, Tsinghua University;Henan Tailong Pharmaceutical Co., Ltd.;

摘要(Abstract):

黄芩为唇形科黄芩属植物黄芩Scutellaria baicalensis的干燥根,是常见大宗中药材之一。市场上,产地、生长年限和采收期等因素,导致黄芩药材质量参差不齐。《中国药典》2020年版规定黄芩药材中黄芩苷≥9%,仅能作为药材质量合格的评判标准,无法判别黄芩药材的优劣。为提升黄芩药材质量控制方法,该研究采用植物代谢组学技术对不同生长年限的黄芩代谢物累积情况进行分析,鉴定得到28种随生长年限(1~3年)增长的代谢物,并通过三重四极杆质谱(UPLC-QQQ-MS)对其中14种主要代谢物进行定量分析,将含量较高、活性较好的黄芩苷、汉黄芩苷、黄芩素和汉黄芩素作为黄芩药材质量评价指标成分。随后,利用高效液相色谱法(HPLC)对32批不同产地、采收期、生长年限黄芩药材的4个指标成分进行含量测定。结果表明,生长年限对黄芩指标成分含量影响较大,一年生样品中指标成分总量最低,二年生最高,三、四年生较二年生总量有所降低。基于HPLC含量测定数据和企业验证结果,推荐黄芩苷≥12.0%、汉黄芩苷≥2.3%、黄芩素≥0.1%、汉黄芩素≥0.03%作为黄芩药材优质标准,为提升黄芩药材质量提供依据。
Scutellariae Radix(SR), derived from the dried root of Scutellaria baicalensis in the family Lamiaceae, commonly serves as Chinese medicinal material. Affected by producing areas, growing years, and harvesting periods, the quality of SR fluctuates in the market. However, baicalin≥9% in SR required in the Chinese Pharmacopoeia(2020 edition) can only determine the qualified SR but cannot identify high-quality SR. To improve the quality control methods of SR, the present study analyzed the accumulation of metabolites in SR of different growth years by plant metabolomics, and identified 28 metabolites increasing with growth years(1-3 years). Subsequently, 14 main metabolites were quantitatively analyzed by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry(UPLC-QQQ-MS). Among them, baicalin, wogonoside, baicalein, and wogonin with high content and good activity were selected as the index components of SR for quality evaluation. A high-performance liquid chromatography(HPLC) method was established to determine the content of four index components in 32 batches of SR from different producing areas, harvesting perio-ds, and growth years. The results showed that the growth years could greatly affect the content of index components. The total content of four index components in 2-year SR was the highest, followed by the 3-/4-year SR and 1-year SR. Based on HPLC data and verification results by enterprises, baicalin ≥12.0%, wogonoside ≥2.3%, baicalein ≥0.1%, and wogonin ≥0.03% were proposed as the evaluation criteria for the high-quality SR. The findings of this study are expected to provide a basis for improving the quality of SR.

关键词(KeyWords): 黄芩;植物代谢组学;指标成分;优质标准
Scutellariae Radix;plant metabolomics;index components;high quality standard

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 张娜;苏风山;周园涛;高启国;陈军力;徐晓丽;韩玲玉;
ZHANG Na;SU Feng-shan;ZHOU Yuan-tao;GAO Qi-guo;CHEN Jun-li;XU Xiao-li;HAN Ling-yu;Traditional Chinese Medicine Institute, School of Pharmaceutical Sciences, Tsinghua University;Henan Tailong Pharmaceutical Co., Ltd.;

DOI: 10.19540/j.cnki.cjcmm.20211123.203

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