卞娅;刘孟生;张丽媛;李国川;冯建明;梁夏;郑玉光;王靓;
BIAN Ya;LIU Meng-sheng;ZHANG Li-yuan;LI Guo-chuan;FENG Jian-ming;LIANG Xia;ZHENG Yu-guang;WANG Jing;Hebei University of Chinese Medical;Hebei Medical University;

• 1:河北中医学院
• 2:河北医科大学

摘要(Abstract)：

为探索射干、鸢尾非药用部位叶片的有效成分物质基础及抗炎活性,首次建立同时测定射干根、鸢尾根、以及不同方法干燥的射干叶和鸢尾叶中鸢尾苷,野鸢尾苷,鸢尾黄素,野鸢尾黄素,次野鸢尾黄素,白射干素6种抗炎活性成分含量的高效液相色谱法,并将上述乙醇提取液进行小鼠耳肿胀实验对其抗炎作用进行比较研究。高效液相色谱法采用Wonda Sil^?C_(18)-WR(4.6 mm×250 mm,5μm)色谱柱,流动相选用乙腈-0.1%磷酸水溶液体系,检测波长265 nm,流速1.0 mL·min?C_(18)-WR(4.6 mm×250 mm,5μm)色谱柱,流动相选用乙腈-0.1%磷酸水溶液体系,检测波长265 nm,流速1.0 mL·min^(-1)的条件下进行梯度洗脱。结果显示射干、鸢尾的根茎和叶片中化学成分种类相似,但含量有一定差别。动物实验结果表明,射干和鸢尾根茎、叶片提取物的高、中、低浓度计量组均对二甲苯致小鼠耳肿胀有抑制作用,证明其具有抗炎作用,为射干、鸢尾非药用部位的开发打下物质及活性研究基础。
In order to explore the anti-inflammatory activity and active ingredient basis from the leaves of the Belamcanda chinensis and Iris tectorum,we established an HPLC method for simultaneous determination of six anti-inflammatory active ingredient contents in the root of the B.chinensis and I.tectorum as well as their leaves with different dry methods,and the anti-inflammatory effects of the extract were studied by the mouse ear swelling experiment.The HPLC analysis was performed on an Agilent Wonda Sil(-1)的条件下进行梯度洗脱。结果显示射干、鸢尾的根茎和叶片中化学成分种类相似,但含量有一定差别。动物实验结果表明,射干和鸢尾根茎、叶片提取物的高、中、低浓度计量组均对二甲苯致小鼠耳肿胀有抑制作用,证明其具有抗炎作用,为射干、鸢尾非药用部位的开发打下物质及活性研究基础。
In order to explore the anti-inflammatory activity and active ingredient basis from the leaves of the Belamcanda chinensis and Iris tectorum,we established an HPLC method for simultaneous determination of six anti-inflammatory active ingredient contents in the root of the B.chinensis and I.tectorum as well as their leaves with different dry methods,and the anti-inflammatory effects of the extract were studied by the mouse ear swelling experiment.The HPLC analysis was performed on an Agilent Wonda Sil^?C_(18)-WR(4.6 mm×250 mm,5 μm),with isocratic elution of acetonitrile-0.1% ortho-phosphoric acid solution at a flow rate of 1.0 mL·min?C_(18)-WR(4.6 mm×250 mm,5 μm),with isocratic elution of acetonitrile-0.1% ortho-phosphoric acid solution at a flow rate of 1.0 mL·min^(-1) and the detection was carried out at 265 nm.The chemical compositions of the B.chinensis and I.tectorum are similar but the contents of them are obviously different.Both rhizome and leaf extract of B.chinensis and I.tectorum had inhibitory effects on inflamed mice induced by dimethylbenzene and had anti-inflammatory effects by animal experiment,which could lay the material and active foundation for the development of the non-medicinal parts of the B.chinensis and I.tectorum.

关键词(KeyWords)： 射干;鸢尾;含量测定;抗炎作用
Belamcanda chinensis;Iris tectorum;content determination;pharmacological effect

Abstract:

Keywords:

基金项目(Foundation): 河北省现代农业技术体系中国药材产业创新团队建设项目(2017041458-2)

作者(Authors): 卞娅;刘孟生;张丽媛;李国川;冯建明;梁夏;郑玉光;王靓;
BIAN Ya;LIU Meng-sheng;ZHANG Li-yuan;LI Guo-chuan;FENG Jian-ming;LIANG Xia;ZHENG Yu-guang;WANG Jing;Hebei University of Chinese Medical;Hebei Medical University;

DOI: 10.19540/j.cnki.cjcmm.20171027.006

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