杨素德;王伟;李家春;萧伟;
YANG Su-de;WANG Wei;LI Jia-chun;XIAO Wei;Jangsu Kanion Pharmaceutical Co. ,Ltd.;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process;

• 1:江苏康缘药业股份有限公司
• 2:中药制药过程新技术国家重点实验室

摘要(Abstract)：

该文建立苁蓉总苷胶囊的HPLC指纹图谱,为其质量评价提供依据。以毛蕊花糖苷为参照物,采用高效液相色谱法,对苁蓉总苷胶囊进行了指纹图谱分析。色谱条件:Waters C18色谱柱(4.6 mm×150 mm,5μm);流动相甲醇-0.1%甲酸溶液梯度洗脱;流速1.0 m L·min-1;检测波长330 nm;柱温30℃。该方法精密度高、重复性好,所测样品中的13个成分均达到基线分离,并且15批成品指纹图谱相似度在0.95以上。该方法准确、可行,是一种简便、有效的中药质量评价手段。
HPLC fingerprint of Congrong Zonggan capsule was established in order to provide basis for quality evaluation. With acteoside as the reference,HPLC was adopted for fingerprint analysis on Congrong Zonggan capsule. The chromatographic conditions were as follows. Waters C18column( 4. 6 mm × 150 mm,5 μm) was used,with methyl alcohol-0. 1% formic acid as the mobile phase for gradient elution at the flow rate of 1. 0 m L·min- 1. The detection wavelength was 330 nm,and the column temperature was 30 ℃.This method was highly accurate and reproducible. All of the 13 components in tested samples reached the baseline resolved peak,and15 batches of finished products showed the similarity of above 0. 95. The method was accurate and feasible and could be used as a simple and effective method to evaluate the quality of the traditional Chinese medicines.

关键词(KeyWords)： 苁蓉总苷胶囊;高效液相色谱法;指纹图谱;松果菊苷
Congrong Zonggan capsule;HPLC;fingerprint;echinacoside

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 杨素德;王伟;李家春;萧伟;
YANG Su-de;WANG Wei;LI Jia-chun;XIAO Wei;Jangsu Kanion Pharmaceutical Co. ,Ltd.;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process;

Email:

DOI:

参考文献(References)：

• [1]袁萍,高俊飞,袁晓.HPLC-DAD测定虎杖中八种成分含量及指纹图谱研究[J].中国现代应用药学,2013,30(1):20.
• [2]杜义龙,李艳荣,张晓峰,等.承德产山楂叶的HPLC指纹图谱研究[J].中国现代应用药学,2012,29(10):906.
• [3]吴晓春,史颖.肉苁蓉的研究与临床应用[J].甘肃中医,2007,20(12):49.
• [4]郝媛媛,岳利君,康建军,等.“沙漠人参”肉苁蓉和锁阳研究进展[J].草业学报,2012,21(2):286.
• [5]中国药典.一部[S].2010:126.
• [6]屠鹏飞,出山武,张正高.肉苁蓉类生药中乙醇苷类成分的RP-HPLC分析[J].药学学报,1997,32(4):294.
• [7]Xiong Q B,Tezuba Y,Kaneko T,et al.Inhibition on nitric oxide by phenylethanoids in activated macrophages[J].Eur J Pharmacol,2000,400(1):137.
• [8]张雷红,堵年生.肉苁蓉化学成分研究概况[J].中成药,2003,25(4):323.
• [9]秦冬梅,胡利萍,谭勇,等.HPLC法测定欧亚列当中松果菊苷的含量[J].中国药房,2011,22(23):2175.
• [10]张彦红,朱磊,梁伟雄.苁蓉总苷胶囊治疗髓海不足型轻中度血管性痴呆40例[J].中医研究,2012,25(6):41.
• [11]朱旭江,贺军权,杜兴,等.兰州肉苁蓉HPLC指纹图谱研究[J].中国药房,2011,47(22):4477.
• [12]吴涛,张娟,卿德刚,等.管花肉苁蓉HPLC指纹图谱研究[J].新疆农业科学,2010,47(3):499.