中国中药杂志

2021, v.46(20) 5260-5269

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几种滇产龙胆属Gentiana药用植物的DNA超级条形码研究
DNA super-barcoding of several medicinal species in Gentiana from Yunnan province

胡海粟;张德全;
HU Hai-su;ZHANG De-quan;College of Pharmacy, Dali University;Institute of Materia Medica, Dali University;

摘要(Abstract):

龙胆属Gentiana为龙胆科中一个具有重要药用价值但种属比较复杂的类群。该属包含众多药用植物种类,其物种鉴定比较困难。该研究利用Illumina Hisep 2500测序平台对几种滇产龙胆属药用植物(滇龙胆草G.rigescens、红花龙胆G.rhodantha和微籽龙胆G.delavayi)进行二代测序,经组装、注释得到3条完整的叶绿体全基因组序列,并结合已发表的粗茎秦艽G.crassicaulis数据,对DNA超级条形码在龙胆属中的物种鉴定进行初步研究。研究发现,滇龙胆草、红花龙胆、微籽龙胆的叶绿体全基因组总长相对较短(分别为146 944、148 992、148 796 bp),共注释得到114个基因,包括78个蛋白编码基因、30个tRNA基因、4个rRNA基因及2个假基因,其叶绿体基因组比较保守。同时,以叶绿体全基因组作为DNA超级条形码,对4种滇产龙胆属药用植物进行物种鉴定。结果表明,这几种龙胆属植物均聚为明显的单系分支,且具有较高的支持率(100%)。这说明DNA超级条形码,在龙胆属这类复杂类群的物种鉴定上具有较大优势。该研究结果将为龙胆属药用植物的准确鉴定、开发利用及其资源保护提供科学依据。
Gentiana is an important but complicated group in Gentianaceae. The genus covers numerous medicinal plants which are difficult to be identified. In the present study, several medicinal species in Gentiana from Yunnan province, including G. rigescens, G.rhodantha, and G. delavayi, were sequenced using the Illumina HiSeq 2500 system. Three complete chloroplast genome sequences were obtained after assembly and annotation. According to several published genome sequences of G. crassicaulis, the DNA super-barcoding of species in Gentiana was preliminarily carried out. The results revealed that chloroplast genomes of the three species were conservative with short lengths(146 944, 148 992, and 148 796 bp, respectively). The genomes encoded 114 genes, including 78 protein-coding genes, 30 tRNA genes, 4 rRNA genes, and 2 pseudogenes. Furthermore, these medicinal species in Yunnan province were identified using DNA super-barcoding based on chloroplast genomes. The results showed that the Gentiana species could be gathered into monophyletic branches with a high support value(100%). It indicated that DNA super-barcoding possessed obvious advantages in discriminating species in complicated genera. This study is expected to provide a scientific basis for the identification, utilization, and conservation of Gentiana species.

关键词(KeyWords): 龙胆属;药用植物;叶绿体全基因组;DNA超级条形码;物种鉴定
Gentiana;medicinal species;complete chloroplast genome;DNA super-barcoding;species identification

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(32060091,31660081);; 云南省教育厅科学研究基金项目(2021Y402)

作者(Author): 胡海粟;张德全;
HU Hai-su;ZHANG De-quan;College of Pharmacy, Dali University;Institute of Materia Medica, Dali University;

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