中国中药杂志

2019, v.44(10) 2051-2058

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不同产地葛根饮片的UPLC指纹图谱结合多成分含量测定研究
Quality research of Puerariae Lobatae Radix from different habitats with UPLC fingerprint and determination of multi-component content

黄紫炎;沈钱能;李平;苏联麟;陈丽红;陆兔林;毛春芹;
HUANG Zi-yan;SHEN Qian-neng;LI Ping;SU Lian-lin;CHEN Li-hong;LU Tu-lin;MAO Chun-qin;School of Pharmacy,Nanjing University of Chinese Medicine;

摘要(Abstract):

建立不同产地葛根饮片的UPLC指纹图谱和同时测定6种异黄酮类化合物含量的UPLC分析方法。采用Waters ACQUITY UPLC BEH C_(18)色谱柱(2. 1 mm×50 mm,1. 7μm),流动相乙腈-0. 05%甲酸,梯度洗脱;检测波长250 nm;流速0. 2 mL·min~(-1);柱温30℃;进样量2μL;采用中药指纹图谱相似度评价系统进行相似度评价,并使用Simca-P软件中的主成分分析(PCA)与偏最小二乘法判别(OPLS-DA)寻找3个产地的差异成分。29批样品相似度均在0. 90以上,表明样品一致性良好,经PCA与OPLS-DA分析,葛根聚集为3组,并发现了葛根芹菜糖苷、大豆苷、大豆苷元等6个差异成分。对其中3'-羟基葛根素、葛根素、3'-甲氧基葛根素、葛根芹菜糖苷、大豆苷、大豆苷元6种异黄酮的含量测定结果表明,同一成分在各产地葛根饮片中的含量差异明显,不同成分的波动范围不同;各产地6种异黄酮质量分数为安徽11. 21%>河南10. 97%>陕西9. 38%。构建的不同产地葛根饮片UPLC指纹图谱结合多成分含量测定方法为完善葛根饮片的质量控制和品质评价提供了更全面的参考。
To establish ultra performance liquid chromatography( UPLC) fingerprint of Puerariae Lobatae Radix from different habitats and simultaneously determine the contents of six isoflavonoids. The UPLC fingerprint analysis and content determination were performed on a Waters ACQUITY UPLC BEH C_(18)( 2. 1 mm×50 mm,1. 7 μm) chromatographic column,with acetonitrile-0. 05% formic acid as mobile phase for gradient elution. The detection wavelength was set at 250 nm; the flow rate was 0. 2 mL·min~(-1); the column temperature was 30 ℃ and the injection volume was 2 μL. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( TCM) was adopted; principal component analysis( PCA) and discriminant analysis by partial least square method( PLS-DA) in Simca-P software were used to identify the differential components in samples from three habitats. The similarity was over 0. 90 in 29 batches of samples,indicating good consistency of the samples. The samples were clustered into 3 categories by PCA and PLS-DA,and six differential components such as puerarin apioside,daidzin,and isoflavoues aglycone were found. The determination results of 6 isoflavones,including 3'-hydroxy puerarin,puerarin,3'-methoxy puerarin,puerarin apioside,daidzin,and isoflavoues aglycone,showed that the content of the same component and the fluctuation range between different components were all different among different habitats. The total content of 6 isoflavones from different regions was Anhui 11. 21% >Henan 10. 97% >Shannxi 9. 38%. The establishment of UPLC fingerprint combined with simultaneous determination of 6 active components provides a more comprehensive reference for quality control and quality evaluation of Puerariae Lobatae Radix.

关键词(KeyWords): 葛根;UPLC指纹图谱;多成分含量测定;主成分分析;偏最小二乘法判别分析
Puerariae Lobatae Radix;UPLC fingerprint;determination of multi-component content;principal component analysis;discriminant analysis of partial least square method

Abstract:

Keywords:

基金项目(Foundation): 国家公益性行业科研专项(201307008)

作者(Author): 黄紫炎;沈钱能;李平;苏联麟;陈丽红;陆兔林;毛春芹;
HUANG Zi-yan;SHEN Qian-neng;LI Ping;SU Lian-lin;CHEN Li-hong;LU Tu-lin;MAO Chun-qin;School of Pharmacy,Nanjing University of Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20190116.002

参考文献(References):

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