中国中药杂志

2018, v.43(01) 65-71

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西洋参不同组织部位中皂苷生物合成相关基因的表达研究
Expression of saponin biosynthesis related genes in different tissues of Panax quinquefolius

王康宇;刘伟灿;张美萍;赵明珠;王艳芳;李俐;孙春玉;胡可心;丛悦怡;王义;
WANG Kang-yu;LIU Wei-can;ZHANG Mei-ping;ZHAO Ming-zhu;WANG Yan-fang;LI Li;SUN Chun-yu;HU Ke-xin;CONG Yue-yi;WANG Yi;College of Life Science,Jilin Agricultural University;Research Center for Ginseng Genetic Resources Development and Utilization;College of Traditional Chinese Medicine,Jilin Agricultural University;

摘要(Abstract):

通过皂苷含量与基因表达量之间相关性分析,获得西洋参中不同组织部位皂苷含量与人参皂苷生物合成相关基因表达之间联系。以四年生红果期西洋参的14个组织部位为材料,利用高效液相法和香草醛-硫酸比色法测定西洋参样本中6种单体皂苷(人参皂苷Rg1,Re,Rb1,Rc,Rb2,Rd),分组皂苷和总皂苷含量;同时,利用实时荧光定量PCR法检测西洋参不同组织部位中7种(SQS,OSC,DS,β-AS,SQE,P450,FPS)人参皂苷生物合成相关基因的表达量。在西洋参中7种人参皂苷生物合成相关酶基因虽然参与人参皂苷合成,但是β-AS和P450基因的表达对单体皂苷,分组皂苷及总皂苷含量影响不显著;FPS,SQS,OSC,DS和SQE这5种基因表达对单体皂苷Re,Rg1,Rb1,Rd,分组皂苷PPD和PPT,单体总皂苷TMS和总皂苷TS含量影响显著或者极显著(P<0.05或P<0.01)。西洋参中部分单体皂苷,分组皂苷以及总皂苷的生物合成受着皂苷合成途径多种酶基因相互作用影响,生物合成途径中关键酶基因表达差异决定了西洋参中不同组织部位皂苷含量。因此,该研究通过相关性分析进一步明确了FPS,SQS,OSC,DS和SQE这5种酶基因是控制西洋参中皂苷合成的关键酶,它们通过集群协同表达互作的方式调控西洋参中人参皂苷的生物合成。
The relationship between saponin content of Panax quinquefolius in different parts of the organization and expression of ginsenoside biosynthesis related gene was obtained by the correlation analysis between saponin content and gene expression.The 14 tissue parts of P.quinquefolius were studied,six saponins in P.quinquefolius.Samples(ginsenoside Rg1,Re,Rb1,Rc,Rb2 and Rd),group saponins and total saponins were determined by high performance liquid chromatography and vanillin-sulfuric acid colorimetric method.Simultaneously,the expression levels of 7 ginsenoside biosynthesis related genes(SQS,OSC,DS,β-AS,SQE,P450 and FPS) in different tissues of P.quinquefolius were determined by Real-time fluorescence quantitative PCR.Although 7 kinds of ginsenoside biosynthesis related enzyme gene in the P.quinquefolius involved in ginsenoside synthesis,the expression of β-AS and P450 genes had no significant effect on the content of monosodium saponins,grouping saponins and total saponins,FPS,SQS,OSC,DS and SQE had significant or extremely significant on the contents of single saponins Re,Rg1,Rb1,Rd,group saponin PPD and PPT,total saponin TMS and total saponin TS(P<0.05 or P<0.01).The biosynthesis of partial saponins,grouping saponins and total saponins in P.quinquefolius was affected by the interaction of multiple enzyme genes in the saponin synthesis pathway,the content of saponins in different tissues of P.quinquefolius was determined by the differences in the expression of key enzymes in the biosynthetic pathway.Therefore,this study further clarified that FPS,SQS,OSC,DS and SQE was the key enzyme to control the synthesis of saponins in P.quinquefolius by correlation analysis,the biosynthesis of ginsenosides in P.quinquefolius was regulated by these five kind of enzymes in cluster co-expression of interaction mode.

关键词(KeyWords): 西洋参;基因表达;人参皂苷生物合成途径;相关性;实时荧光定量PCR
Panax quinquefolius;gene expression;ginsenoside biosynthesis pathway;correlation;real-time quantitative PCR

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划(863)项目(2013AA102604-3);; 吉林省发改委-吉林省省级产业创新专项(2016C04);; 吉林省科技厅自然科学基金主题引导项目(20170101010JC);; 吉林农业大学博士科研启动基金项目(201618)

作者(Author): 王康宇;刘伟灿;张美萍;赵明珠;王艳芳;李俐;孙春玉;胡可心;丛悦怡;王义;
WANG Kang-yu;LIU Wei-can;ZHANG Mei-ping;ZHAO Ming-zhu;WANG Yan-fang;LI Li;SUN Chun-yu;HU Ke-xin;CONG Yue-yi;WANG Yi;College of Life Science,Jilin Agricultural University;Research Center for Ginseng Genetic Resources Development and Utilization;College of Traditional Chinese Medicine,Jilin Agricultural University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20171030.006

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