孙维广;柯雪红;李苑;花汝凤;陈锦富;
SUN Wei-guang1,KE Xue-hong2,LI Yuan3,HUA Ru-feng2,CHEN Jin-fu2 (1.Guangzhou Xingqun Pharmaceutical Ltd.Co.,Guangzhou 510288,China;2.First Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China;3.Provincial Institute of Medicine,Guangzhou 510440,China)

• 1:广州星群药业股份有限公司
• 2:广州中医药大学第一附属医院实验中心
• 3:广东省药物研究所

摘要(Abstract)：

目的:建立夏枯草HPLC指纹图谱,为中药材质量控制提供有效的方法。方法:Sunfire C18色谱柱;A相为1%醋酸,B相为甲醇组成的梯度洗脱液;检测波长290 nm;柱温30℃;流速1.0 mL.min-1;分析时间60 min。对不同产地的夏枯草指纹图谱测定,采用统计方法对其进行分类。结果:10批夏枯草指纹图谱相似度≥0.810。采用聚类分析将不同产地的药材分为3类。结论:该方法稳定可靠,可以有效地用于夏枯草原材料质量控制,为制剂的稳定性提供参考。
Objective: To establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.Method: A sunfire C18 analytical column was used.The mobile phase A was 1% acetic acid,and mobile phase B was methanol.The elution was in gradient mode and detection wavelengfh was set at 290 nm.The flow rate was 1.0 mL·min-1 and the column temperature at 30 ℃.The analysis time was 60 min.Result: The similarity of 10 batches of P.vulgaris was not lower than 0.810.The fingerprints of the herbal medicine were classified P.vulgaris on the results of cluster analysis.Conclusion: This method is available for quality evaluation and control the quality of P.vulgaris.

关键词(KeyWords)： 夏枯草;指纹图谱;HPLC;质量控制
Prunella vulgaris;fingerprint;HPLC;quality control

Abstract:

Keywords:

基金项目(Foundation): 广州市科技攻关重大项目(2007Z1-E5033)

作者(Author): 孙维广;柯雪红;李苑;花汝凤;陈锦富;
SUN Wei-guang1,KE Xue-hong2,LI Yuan3,HUA Ru-feng2,CHEN Jin-fu2 (1.Guangzhou Xingqun Pharmaceutical Ltd.Co.,Guangzhou 510288,China;2.First Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China;3.Provincial Institute of Medicine,Guangzhou 510440,China)

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参考文献(References)：

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