费曜;钟国跃;蒋伟;
FEI Yao;ZHONG Guo-yue;JIANG Wei;Integrated Department of Chinese Medicine,Chongqing Medical University;Jiangxi University of Traditional Chinese Medicine;

• 1:重庆医科大学中医药学院
• 2:江西中医药大学

摘要(Abstract)：

建立常用藏药"松蒂"(蓖齿虎耳草)HPLC指纹图谱,为"松蒂"质量控制提供有效方法。采用Amethyst-C18-P色谱柱(4.6 mm×250 mm,5μm);甲醇-0.4%甲酸水溶剂系统梯度洗脱;检测波长254 nm;柱温30℃;流速为1.0 mL·min-1。对不同来源和不同批次的蓖齿虎耳草指纹图谱测定,采用"中药色谱指纹图谱相似度评价系统2004A版"软件进行模式分析、共有峰确定及相似度计算,并根据共有峰对10批样品进行SPSS聚类分析。各批次样品成分类型基本一致,标定了10个共有峰,黄酮和皂苷为蓖齿虎耳草的主要化学成分。但指纹图谱特征峰数量和峰面积受采集地、样品来源方式影响较大,来源于同一地区品种准确性和纯净度较高的样品相似度较高。该方法简便准确,灵敏度高,重复性好,可以作为"松蒂"(蓖齿虎耳草)的质量控制方法。
The research was carried out to establish HPLC fingerprints of Tibetan medicinal herb ″Songdi″(Saxifraga umbellulata var. pectinata),and to provide reference for identification an quality control of it. It was performed on an Amethyst-C18-P(4. 6mm × 250 mm,5 μm)column with the mobile phase of methanol-0. 4% formic acid in a linear gradient mode at a flow rate of 1. 0 mL·min-1. The column temperature was 30 ℃,and the detection wavelength was set at 254 nm. The software for chromatographic fingerprint was applied to analyse the pattern analysis,the common peaks and similarity. Cluster analysis was done based on the common peaks data of 33 samples from different plant species and sources by SPSS software. Ten common chromatographic peaks were identified by fingerprint,showing a low similarity in constituent and variety. Flavonoids and saponins were the principal components. The number and area of peaks were affected by the collection sources and method. The high similarity are showed by the samples derived from the same area with high accuracy and high purity. The method is so simple,exclusive,stable and high repeatable that it can provide reference for identification and quality assessment of ″Songdi″(S. umbellulata var. pectinata).

关键词(KeyWords)： 藏药;松蒂;蓖齿虎耳草;HPLC指纹图谱;聚类分析
Tibetan medicinal herb;Songdi;Saxifraga umbellulata var.pectinata;HPLC fingerprints;systematically cluster analysis

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2006DAI06A11-12)

作者(Author): 费曜;钟国跃;蒋伟;
FEI Yao;ZHONG Guo-yue;JIANG Wei;Integrated Department of Chinese Medicine,Chongqing Medical University;Jiangxi University of Traditional Chinese Medicine;

Email:

DOI:

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