中国中药杂志

2013, v.38(10) 1513-1517

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气滞胃痛颗粒全时段多波长融合指纹图谱研究及多成分定量分析
Study on all-time multi-wavelength fusion fingerprint of Qizhiweitong granules and multi-component quantitative analysis

姚东;孟宪生;王帅;包永睿;潘英;韩凌;
YAO Dong1,MENG Xian-sheng1,WANG Shuai1,BAO Yong-rui1,PAN Ying2,HAN Ling2(1.College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China; 2.Liaoning Benxi Sanyao Co.,Ltd.,Benxi 117004,China)

摘要(Abstract):

目的:采用全时段多波长融合指纹定量为主要技术手段,对气滞胃痛颗粒进行质量控制。方法:Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相0.02%甲酸溶液-乙腈,线性梯度洗脱,流速1.0 mL.min-1,柱温30℃,检测器波长230,254,283 nm,使用Matlab软件编程,对dif格式数据进行全时段多波长融合。结果:白芍药苷在56.5~452 mg.L-1,芍药苷在107~856 mg.L-1,甘草苷在73.4~687 mg.L-1,柚皮苷在109~872 mg.L-1,新橙皮苷在48.0~384 mg.L-1,甘草酸盐在38.6~308 g.mL-1线性关系良好,r均为0.999 8。结论:该方法简便、准确,重复性好,可以为气滞胃痛颗粒的质量控制提供依据。
Objective: To control the quality of Qizhiweitong granules with the all-time multi-wavelength fusion fingerprint quantification as the major technique.Method: Agilent TC-C18(4.6 mm×250 mm,5 μm) chromatographic column was adopted,with 0.02% formic acid water-acetonitrile as the mobile phase for linear gradient elution.The flow rate was 1 mL·min-1,column temperature was 30 ℃,and detector wavelength was 230,254,283 nm.Matlab was adopted for all-time multiple-wavelength fusion for data in dif format.Result: A good relationship was shown for albiflorin in 56.5-452 mg·L-1(r=0.999 8),paeoniflorin in 107-856 mg·L-1(r=0.999 8),licorice glycoside in 73.4-687 mg·L-1(r=0.999 8),naringin in 109-872 mg·L-1(r=0.999 8),neohesperidin in 48.0-384 mg·L-1(r=0.999 8),and glycyrrhizic acid in 38.6-308 mg·L-1(r=0.999 8),with recoveries of 0.999 8.Conclusion: The method is simple,accurate and highly reproducible,and can provide basis for quality control of Qizhiweitong granules.

关键词(KeyWords): 气滞胃痛颗粒;指纹图谱;全时段多波长融合;多指标成分控制
Qizhiweitong granules;fingerprint;all-time multi-wavelength fusion;multi-index composition control

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2010ZX09401-304-201)

作者(Author): 姚东;孟宪生;王帅;包永睿;潘英;韩凌;
YAO Dong1,MENG Xian-sheng1,WANG Shuai1,BAO Yong-rui1,PAN Ying2,HAN Ling2(1.College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China; 2.Liaoning Benxi Sanyao Co.,Ltd.,Benxi 117004,China)

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