中国中药杂志

2020, v.45(12) 2872-2880

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基于质谱和多元统计分析探讨升炼前后龟龄集化学组成的差异
Study on difference of chemical compositions of Guilingji before and after alchemy based on mass spectrometry and multivariate statistical analysis

史静超;高晓霞;张爱荣;张斌;秦雪梅;杜冠华;
SHI Jing-chao;GAO Xiao-xia;ZHANG Ai-rong;ZHANG Bin;QIN Xue-mei;DU Guan-hua;Modern Research Center for Traditional Chinese Medicine, Shanxi University;Department of Traditional Chinese Pharmacy, Shanxi University of Traditional Chinese Medicine;Shanxi Guangyuyuan Chinese Medicine Co., Ltd.;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College;

摘要(Abstract):

采用超高效液相色谱-四极杆-静电场轨道阱串联质谱和电感耦合等离子体质谱结合多元统计分析,对龟龄集升炼前后有机化学成分和无机元素的变化规律进行了探究,发现龟龄集升炼前后差异有机成分共39个,鉴定了其中24个化合物,差异成分包括紫罗兰酮、查尔酮、酰胺、脂肪酸类化合物,其含量在升炼后升高;还有黄酮、异黄酮、二氢黄酮、黄酮苷、香豆素类化合物,其含量在升炼后降低。升炼前后龟龄集中差异无机元素为硼(B)、硅(Si)、镁(Mg)、钾(K)、铬(Cr)、镍(Ni),其中B,Si升炼后含量降低,Mg,K,Cr,Ni含量升高。升炼后物质含量的变化改变了复方作用于机体的寒热药性,表现为燥性降低,由热性转温、平性;改善了复方药物组分的膜通透性和吸收作用。该研究初步探明了龟龄集升炼前后化学成分的变化及对复方药物组分理化性质和药性的影响,为阐明升炼工艺的科学内涵奠定了研究基础。
UPLC-Q-Orbitrap MS/MS and ICP-MS coupled with multivariate statistical analysis was employed to explore the differences in chemical compositions of Guilingji(GLJ) before and after alchemy.The changes in organic chemical compositions and inorganic elements were observed and 39 differential organic compositions were found in GLJ after alchemy, 24 compounds of which were identified. The differential compositions of GLJ included violet ketones, chalcones, amides, and fatty acids whose contents were increased after alchemy, as well as flavones, isoflavones, dihydroflavones, flavonoid glycosides, and coumarins whose content were decreased after alchemy. This study showed 6 inorganic elements filtered out as markers for distinguishing GLJ before and after alchemy, including B, Si, Mg, K, Cr, and Ni.The contents of Mg, K, Cr and Ni were increased while the contents of B and Si were decreased after alchemy.The difference of the contents after alchemy changed the cold and hot properties of the compound, showing the decrease of dryness, and the hot property was changed to warm and neutral properties; in addition, the membrane permeability and absorption of the compound compositions were improved. In this study, we preliminarily investigated the changes of chemical compositions in GLJ before and after alchemy as well as the effects of alchemy on physical and chemical properties and cold-heat nature of GLJ, laying a foundation for further clarifying the scientific connotation of alchemy process.

关键词(KeyWords): 龟龄集;升炼工艺;超高效液相色谱-四极杆-静电场轨道阱串联质谱;电感耦合等离子体质谱;多元统计分析
Guilingji;alchemy;UPLC-Q-Orbitrap MS/MS;ICP-MS;multivariate statistical analysis

Abstract:

Keywords:

基金项目(Foundation): 山西省重点研发计划重点项目(201603D3003006)

作者(Author): 史静超;高晓霞;张爱荣;张斌;秦雪梅;杜冠华;
SHI Jing-chao;GAO Xiao-xia;ZHANG Ai-rong;ZHANG Bin;QIN Xue-mei;DU Guan-hua;Modern Research Center for Traditional Chinese Medicine, Shanxi University;Department of Traditional Chinese Pharmacy, Shanxi University of Traditional Chinese Medicine;Shanxi Guangyuyuan Chinese Medicine Co., Ltd.;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College;

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