不同产地“当归类”药材中藁本内酯含量比较Comparison on content of ligustilides in different Danggui samples
李曦;张丽宏;吕光华;王晓晓;姜卫东;金玉青;赵中振;
LI Xi;ZHANG Li-hong;LV Guang-hua;WANG Xiao-xiao;JIANG Wei-dong;JIN Yu-qing;ZHAO Zhong-zhen;Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica,School of Pharmacy,Chengdu University of Traditional Chinese Medicine;Sichuan Provincial Institute for Food and Drug Control;School of Chinese Medicine,Hong Kong Baptist University;
摘要(Abstract):
为探讨活性成分藁本内酯的含量与"当归类"药材的产地、性状、质量和品种的相关性,应用HPLC-DAD-MS测定了30份不同产地的中国当归,6份日本当归,4份韩国当归和4份欧洲当归样品中Z-藁本内酯和E-藁本内酯的含量,以Z-和E-藁本内酯的含量之和为指标进行样品间比较。中国当归Angelica sinensis中藁本内酯的质量分数为5.63~24.53 mg·g-1,样品间差异较大,平均值为11.02 mg·g-1(n=30)。其中,云南、四川、甘肃和陕西产当归中藁本内酯的质量分数分别为13.90(n=6),12.51(n=6),10.04(n=13),5.631 mg·g-1(n=1);同一省内各县产的当归中藁本内酯的含量也有差异。药材性状以当归小、侧根多而细、断面色黄白、气味浓郁者,藁本内酯的含量高。6份日本当归A.acutiloba和A.acutiloba var.sugiyamae和2份欧当归Levisticum officinale中藁本内酯的质量分数分别为1.00(n=6),2.78 mg·g-1(n=2),为中国当归的9.12%,25.23%。4份韩国当归A.gigas和2份欧洲当归A.archangelica中未检测到藁本内酯。由此可见,不同产地中国当归的性状不同,藁本内酯的含量和药材质量也有差异;中、日、韩和欧洲当归原植物的来源不同,藁本内酯等活性成分有显著差异,其功效也会不同,不宜替代、混淆用药。
Bioactivity of Danggui is linked to the content of ligustilide,but the relationship between ligustilide with herb shape,cultivating areas and plant species is still unknown.The relationship was investigated by quantifying on the amounts of Z-ligustilide and E-ligustilide by HPLC-DAD-MS method,and then comparing the content of ligustilides(the sum of Z-ligustilide and E-ligustilide) among forty-four various " Danggui" samples containing thirty Chinese Danggui(CDG),six Japanese Danggui(JDG),four Korea Danggui(KDG) and four European Danggui(EDG).Results showed that the content of ligustilides in CDG samples(Angelica sinensis) was in the range of 5.63-24.53 mg·g-1with the mean of 11.02 mg·g-1(n =30).Ligustilides amounts were varied among samples cultivated in different areas in China,i.e.13.90 mg·g-1(n =6) in Yannan,12.51 mg·g-1(n =6) in Sichuan and 10.04 mg·g-1(n =13) in Gansu.It was also found that ligustilides content was related to the shape,color and fragrance of herb,e.g.the relative larger amount of ligustilides was in the small main root,long rootlet and perfumed sample.Further,ligustilides contents were estimated to be 1.00 mg·g-1(n =6) in JDG samples(A.acutiloba and A.acutiloba var.sugiyamae) and 2.78 mg·g-1(n =2) in EDG samples(lovage root,Levisticum officinale).However,ligustilides could not be detected in the four KDG samples(A.gigas) and two EDG samples(angelica root,A.archangelica).It has been concluded that ligustilide is significant variant among plant species,which may result in the variety of bioactivity and therapeutic effect.
关键词(KeyWords):
中国当归;日本当归;韩国当归;欧洲当归;藁本内酯;HPLC;含量测定
Angelica spp.;Levisticum officinale;ligustilide;HPLC;content determination
基金项目(Foundation): 国家自然科学基金项目(81073044)
作者(Author):
李曦;张丽宏;吕光华;王晓晓;姜卫东;金玉青;赵中振;
LI Xi;ZHANG Li-hong;LV Guang-hua;WANG Xiao-xiao;JIANG Wei-dong;JIN Yu-qing;ZHAO Zhong-zhen;Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica,School of Pharmacy,Chengdu University of Traditional Chinese Medicine;Sichuan Provincial Institute for Food and Drug Control;School of Chinese Medicine,Hong Kong Baptist University;
Email:
DOI:
参考文献(References):
- [1]中国药典.一部[S].2010:124.
- [2]左爱华,王莉,肖红斌.藁本内酯药理学和药代动力学研究进展[J].中国中药杂志,2012,37(22):3350.
- [3]王祝举,唐力英,赫北湘,等.HPLC测定当归中藁本内酯的含量[J].中国中药杂志,2005,30(21):1699.
- [4]Lu G H,Chan K,Chan C L,et al.Quantification of ligustilides in the roots of Angelica sinensis and related umbelliferous medicinal plants by high-performance liquid chromatography and liquid chromatography-mass spectrometry[J].J Chromatogr A,2004,1046(2):101.
- [5]Lao S C,Li S P,Kan K,et al.Identification and quantification of 13 components in Angelica sinensis(Danggui)by gas chromatography-mass spectrometry coupled with pressurized liquid extraction[J].Anal Chim Acta,2004,526:131.
- [6]日本药局方解说书编集委员会.第十四改正日本药局方[S].东京:株式会社广川书店,2001:2764.
- [7]金镇雄.生药学[M].首尔:东明社,2006:123.
- [8]徐荣培,庐盛洙,李熏揆.当归的等级化研究[J].天津中医药,2009,26(1):81.
- [9]肖培根.新编中药志.第1卷[M].北京:化学工业出版社,2002:425.
- [10]Council of Europe.European pharmacopoeia.Vol.2[S].5 edition.Strasbourg,2004:1003,1932.
- [11]British Pharmacopoeia Commission.Brithish pharmacopoeia.Vol.1[S].London:The Stationery Office,2002:139,1061.
- [12]Barnes J,Anderson L A,Phillipson J D.Herbal Medicines[M].2 edition.London:pharmaceutical Press,:47.
- [13]Lu G H,Chan K,Liang Y Z,et al.Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese angelica from related umbelliferae herbs[J].J Chromatogr A,2005,1073:383.
- [14]Wagner H,Bauer R,Xiao P,et al.Chinese drug monographs and analysis.Vol.3.No.14.[M].Ktzting/Bayer.Wald:Verlag für Ganzheitliche Medizin,2001.
- [15]Lin L Z,He X G,Lian L Z,et al.Liquid chromatographic-electrospray mass spectrometric study of the phthalides of Angelica sinensis and chemical changes of Z-ligustilide[J].J Chromatogr A,1998,810:71.
- [16]李丽丽,刘向前,高敬铭,等.中、日、韩当归中挥发油成分研究和总香豆素含量测定[J].中国药师,2009,12(10):1344.
- [17]Eeva M,Rauha J P,Vuorela P,et al.Computer-assisted,highperformance liquid chromatography with mass spectrometric detection for the analysis of coumarins in Peucedanum palustre and Angelica archangelica[J].Phytochem Anal,2004,15:167.
- 李曦
- 张丽宏
- 吕光华
- 王晓晓
- 姜卫东
- 金玉青
- 赵中振
LI Xi- ZHANG Li-hong
- LV Guang-hua
- WANG Xiao-xiao
- JIANG Wei-dong
- JIN Yu-qing
- ZHAO Zhong-zhen
- Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica
- School of Pharmacy
- Chengdu University of Traditional Chinese Medicine
- Sichuan Provincial Institute for Food and Drug Control
- School of Chinese Medicine
- Hong Kong Baptist University
- 李曦
- 张丽宏
- 吕光华
- 王晓晓
- 姜卫东
- 金玉青
- 赵中振
LI Xi- ZHANG Li-hong
- LV Guang-hua
- WANG Xiao-xiao
- JIANG Wei-dong
- JIN Yu-qing
- ZHAO Zhong-zhen
- Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica
- School of Pharmacy
- Chengdu University of Traditional Chinese Medicine
- Sichuan Provincial Institute for Food and Drug Control
- School of Chinese Medicine
- Hong Kong Baptist University