中国中药杂志

2004, (10)

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川芎嗪逆转HepG2/ADM细胞多药耐药性的体外研究
Study on ligustrazine in reversing multidrug resistance of HepG2/ADM cell in vitro

梅英,石毓君,左国庆,龚建平,刘长安,李旭宏,任孟军
MEI Ying, SHI Yu-jun, ZUO Guo-qing, GONG Jian-ping, LIU Chang -an, LI Xu-hong, REN Meng-jun (The Department of Hepato-biliary Surgery, Seconed Affiliated Hospi tal of Chongqing Medical University, Chongqing 400010, China) [

摘要(Abstract):

目的 :研究川芎嗪对人肝癌多药耐药细胞系HepG2 /ADM多药耐药性的逆转效应及对P gp170表达的影响 ,深入探讨川芎嗪逆转肿瘤细胞多药耐药的机理。方法 :应用细胞培养、细胞毒实验、RT PCR、流式细胞仪等方法 ,观察川芎嗪对体外培养HepG2 /ADM细胞的逆转作用。结果 :川芎嗪可以使细胞膜表面的P gp170表达减弱 ,增加阿霉素对HepG2 /ADM细胞的毒性 ;RT PCR结果表明川芎嗪可以使HepG2 /ADM细胞的mdr1基因表达减弱 ;流式细胞仪检测结果表明川芎嗪可以使HepG2 /ADM细胞内罗丹明 12 3的浓度增加 ,从而增加阿霉素对HepG2 /ADM细胞的毒性。结论 :川芎嗪具有在体外逆转HepG2 /ADM细胞多药耐药的效应 ,具有临床应用前景。
Objective: To study the reverse effec t of ligustrazine (TMP) on HepG2/ADM, a herd of hepatocellular carcinoma cell, mult idurg resistance(MDR) and the influence of P-gp170 expression. Meth od: The reverse effect of ligustrazine on HepG2/ADM cell was observe d, with the methods of cell culture,MTT′s analyze,RT-PCR and Flow cytometri c, etc. Result: Ligustrazine could make MDR of cell lin e of HepG2/ADM reduce the expression of P-gp170, enhance the density of adriam ycin in cell and increase the adriamycin′s cytotoxicity. With the Flow cytome tric, the results of RT-PCR showed the transcriptional activity of the MDR1 de creased. Conclusion:Ligustrazine can reverse MDR of HCC cell line of HepG2/ADM and has prospect in clinical use. [

关键词(KeyWords): 川芎嗪;逆转;多药耐药性
ligustrazine; reversal; multidurg resista nce

Abstract:

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基金项目(Foundation): 重庆市卫生局中医药课题 (渝中医〔2 0 0 2〕 3 9)

作者(Author): 梅英,石毓君,左国庆,龚建平,刘长安,李旭宏,任孟军
MEI Ying, SHI Yu-jun, ZUO Guo-qing, GONG Jian-ping, LIU Chang -an, LI Xu-hong, REN Meng-jun (The Department of Hepato-biliary Surgery, Seconed Affiliated Hospi tal of Chongqing Medical University, Chongqing 400010, China) [

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