赵丹;吴桐宇;关永强;马国需;张晶;石磊岭;
ZHAO Dan;WU Tong-yu;GUAN Yong-qiang;MA Guo-xu;ZHANG Jing;SHI Lei-ling;College of Chinese Medicine Material,Jilin Agricultural University;Xin Jiang Institute of Chinese and Ethnic Medicine;Center of Research and Development on Life Sciences and Environment Sciences,Harbin University of Commerce;

• 1:吉林农业大学中药材学院
• 2:新疆维吾尔自治区中药民族药研究所
• 3:哈尔滨商业大学生命科学与环境研究中心

摘要(Abstract)：

采用柱色谱方法对天山假狼毒Stelleropsis tianschanica根的95%乙醇提取物进行分离纯化,利用波谱技术对分得到的化合物进行结构鉴定。从天山假狼毒中分离得到了17个化合物,分别鉴定为5'-甲氧基落叶松脂醇(1)、松脂素(2)、双白瑞香素(3)、acutissimalignan B(4)、(+)-开环异落叶松树脂酚(5)、(+)-表松脂酚(6)、7-甲氧基西瑞香素(7)、thero-8S-7-methoxysyringylglycerol(8)、1-O-methyl-guaiacylglycerol(9)、2R-22'-阿魏酸酯-2,3-二羟基丙基酯(10)、vesiculosin(11)、4β,5βHguai-9,7(11)-dien-12,8-olide-1α,8α-diol(12)、(-)-nortrachelogenin(13)、4α,5βH-guai-9,7(11)-dien-12,8-olide-1α,8α-diol(14)、罗汉松脂素(15)、落叶松树脂素(16)和异落叶松树脂醇(17)。其中化合物1~13为首次从该属植物中分离得到。采用免疫荧光检测技术化合物3,7,10~14对孤儿核受体TR3的激活作用进行了考察,结果表明在50μmol·L~(-1)下,化合物10的TR3激活率高达76.38%,化合物3,7,11~14的能力较弱,均在50.0%左右。
The constituents from 95% ethanol extract of the roots of Stelleropsis tianschanica were purified by column chromatography techniques,leading to the isolation of 17 compounds. Their structures were elucidated by spectroscopic dataas 5 '-methoxy lariciresinol( 1),pinoresinol( 2),daphnoretin( 3),acutissimalignan B( 4),( +)-secoisolariciresinol( 5),( +)-epipinoresinol( 6),7-methyi-daphnoretin( 7),thero-8 S-7-methoxysyringylglycerol( 8),1-O-methyl-guaiacylglycerol( 9),2 R-22 '-ferulic acid ester-2,3-dihydroxypropyl ester( 10),vesiculosin( 11),4 β,5 βH-guai-9,7( 11)-dien-12,8-olide-1 α,8 α-diol( 12),(-)-nortrachelogenin( 13),4α,5βH-guai-9,7( 11)-dien-12,8-olide-1α,8α-diol( 14),matairesinol( 15),lariciresinol( 16)and isolariciresinol( 17). Among them,compounds 1-13 wereobtained for the first time fromthe genus Stelleropsis. Compounds3,7,10-14 were tested for their activation of orphan nuclear receptor TR3 with the immunofluorescence technology in 50 μmol·L~(-1). The results showed that compound 10 displayed moderate activity with the activity ratio of 76. 38 %,and the others were only about 50. 0 %.

关键词(KeyWords)： 天山假狼毒;化学成分;松脂素;双白瑞香素;TR3
Stelleropsis tianschanica;chemical constituents;pinoresinol;daphnoretin;TR3

Abstract:

Keywords:

基金项目(Foundation): 乌鲁木齐市科学技术计划项目(G161320012);; 自治区卫生计生委青年科技人才专项(2015Y39);; 新疆维吾尔自治区自然科学基金项目(2017D01A72)

作者(Author): 赵丹;吴桐宇;关永强;马国需;张晶;石磊岭;
ZHAO Dan;WU Tong-yu;GUAN Yong-qiang;MA Guo-xu;ZHANG Jing;SHI Lei-ling;College of Chinese Medicine Material,Jilin Agricultural University;Xin Jiang Institute of Chinese and Ethnic Medicine;Center of Research and Development on Life Sciences and Environment Sciences,Harbin University of Commerce;

Email:

DOI: 10.19540/j.cnki.cjcmm.20170728.005

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