中国中药杂志

2011, v.36(10) 1295-1297

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基于复方中药添加剂指纹图谱的多指标成分定量方法研究
Multi-component quantitative method of Chinese herbal additives based on fingerprint

罗娟敏;蔡继宝;肖雪;付婵;廖堃;王义明;罗国安;
LUO Juanmin1,2,CAI Jibao1,XIAO Xue2,FU Chan2,LIAO Kun1,WANG Yiming2,LUO Guoan2(1.Technology Center of China Tobacco Jiangxi industrial Co.,Ltd.,Nanchang 330096,China;2.Analysis Center,Chemistry Department of Tsinghua University,Beijing 100084,China)

摘要(Abstract):

目的:应用HPLC色谱法建立复方中药添加剂的定量指纹图谱。方法:以phenomenex luna C18反相色谱柱为分析柱,0.1%甲酸水溶液-乙腈为流动相,梯度洗脱,全波长扫描。结果:该色谱条件下复方中药添加剂定量指纹图谱具有良好的精密度、重复性、稳定性、回收率。结论:本研究所建立的HPLC方法稳定可靠,简便易行,可用于复方中药添加剂中7种成分的同时定量,为复方中药添加剂的质量评价和质量控制奠定了基础。
Objective: To establish a full-quantified fingerprint method for analyzing Chinese herbal additives by HPLC.Method: A HPLC in combination with PDA detector was applied with a phenomenex luna C18(4.6 mm×250 mm,5 μm) column by gradient elution using acetonitrile-0.1% formic acid solution as the mobile phase.The flow rate was 1 mL·min-1 and the detection wavelength was set at full spectrum scan.Result: According to the selected chromatographic conditions,the full-quantified fingerprint of the Chinese herbal additive has good precision,reproducibility,stability and recovery.Conclusion: The HPLC method developed for simultaneous determination of seven compounds is simple and valid.It can be used for quality evaluation and quality control of Chinese herbal additive and its processing products.

关键词(KeyWords): 复方中药添加剂;定量指纹图谱;高效液相色谱
Chinese herbal additive;full-quantified fingerprint;HPLC

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基金项目(Foundation): 江西中烟工业有限责任公司项目(赣烟工科计2009-14)

作者(Author): 罗娟敏;蔡继宝;肖雪;付婵;廖堃;王义明;罗国安;
LUO Juanmin1,2,CAI Jibao1,XIAO Xue2,FU Chan2,LIAO Kun1,WANG Yiming2,LUO Guoan2(1.Technology Center of China Tobacco Jiangxi industrial Co.,Ltd.,Nanchang 330096,China;2.Analysis Center,Chemistry Department of Tsinghua University,Beijing 100084,China)

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