中国中药杂志

2010, v.35(23) 3178-3183

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吴茱萸药材的HPLC指纹图谱研究
HPLC fingerprint of Euodiae Fructus

赵平;许海玉;许浚;张铁军;朱雪瑜;
ZHAO Ping1,2,XU Haiyu1,2,XU Jun2,ZHANG Tiejun2,ZHU Xueyu2(1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;2.Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China)

摘要(Abstract):

目的:建立吴茱萸药材的HPLC指纹图谱,并对主要成分进行定性定量分析,为科学评价及有效控制其质量提供方法。方法:采用HPLC梯度洗脱法测定10批吴茱萸药材样品的色谱图,应用HPLC-DAD-MS联用技术确定共有峰的归属,并利用相似度评价及系统聚类分析进行模式识别。结果:吴茱萸HPLC指纹图谱有32个共有峰,指认了其中的13个共有峰,并对其中的2种成分进行了定量评价。结论:所建立的HPLC指纹图谱能体现吴茱萸的主要成分,可结合含量测定用于全面控制吴茱萸的质量。
Objective: To establish a HPLC fingerprint for quality evaluation of fructus Euodiae rutaecarpa,and to perform qualitative and quantitative analysis of its main constituents.Method: The 10 batches of samples were analyzed by HPLC linear gradient elution method and evaluated by similarity evaluation and system cluster analysis.The common peaks in chromatographic fingerprint were identified by LC-DAD-MS.Result: The HPLC fingerprint of Euodiae Fructus showed 32 characteristic peaks,among them 13 peaks were identified,and 2 constituents were quantified.Conclusion: The HPLC fingerprint established could reflect the main constituents of Euodiae Fructus,and could combine with content determination to take an overall control of Euodiae Fructus.

关键词(KeyWords): 吴茱萸;HPLC;LC-DAD-MS;指纹图谱
Euodiae Fructus;HPLC;LC-DAD-MS;fingerprint

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2006BAI06A01,2007BAI41B06)

作者(Author): 赵平;许海玉;许浚;张铁军;朱雪瑜;
ZHAO Ping1,2,XU Haiyu1,2,XU Jun2,ZHANG Tiejun2,ZHU Xueyu2(1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;2.Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China)

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