中国中药杂志

2016, v.41(17) 3272-3278

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不同品种枳实HPLC指纹图谱及成分含量差异性研究
Study on HPLC fingerprint and chemical constituent difference of different species of Aurantii Fructus Immaturus

曾鸿莲;刘振丽;宋志前;王淳;董运茁;宁张弛;赵思宇;舒一崧;何丹;王梦蕾;甘嘉荷;刘元艳;
ZENG Hong-lian;LIU Zhen-li;SONG Zhi-qian;WANG Chun;DONG Yun-zhuo;NING Zhang-chi;ZHAO Si-yu;SHU Yi-song;HE Dan;WANG Meng-lei;GAN Jia-he;LIU Yuan-yan;School of Chinese Materia Medica,Beijing University of Chinese Medicine;Institution of Basic Theory,China Academy of Chinese Medical Sciences;

摘要(Abstract):

采用HPLC-DAD方法建立18个批次酸橙枳实以及10个批次甜橙枳实的指纹图谱,通过对照品对照和紫外图谱指认了17个色谱峰并对其进行含量测定。色谱条件:Agilent Poroshell 120 SB-C18色谱柱(4.6 mm×100 mm,2.7μm),以流动相甲醇-0.1%甲酸水溶液梯度洗脱,流速0.6 m L·min-1,检测波长318 nm,柱温为30℃。采用中药色谱指纹图谱相似度评价系统(2004A)软件进行指纹图谱分析,进一步通过SIMCA-P 13.0软件对17个确认的成分含量进行最小二乘法判别分析,确定区分酸橙枳实与甜橙枳实的差异性标记物。该方法具有良好的精密度、稳定性和重复性,为枳实的质量控制和评价提供依据。
This study is to establish an HPLC fingerprint by HPLC-DAD method and simultaneous quantitative analysis of 17 components of 18 batches of Citrus aurantium and 10 batches of C. sinensis. The separation was performed on an Agilent Poroshell 120 SBC18( 4. 6 mm × 100 mm,2. 7 μm) column with the gradient elution of methanol-0. 1% formic acid water,the flow was 0. 6 m L·min~(-1). The detection wavelength was set at 318 nm. The column temperature was maintained at 30 ℃. The data calculation was performed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( Version 2004A) together with SIMCA-P 13. 0 software to clarify the differential marker between these two different species of Aurantii Fructus Immaturus. This method has good precision stability and repeatability that could provide basis for quality control and evaluation of Aurantii Fructus Immaturus.

关键词(KeyWords): HPLC-DAD;酸橙枳实;甜橙枳实;指纹图谱;含量测定;差异性标记物
HPLC-DAD;Citrus aurantium;Citrus sinensis;fingerprint;quantitative analysis;differential marker

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81573569);; 北京中医药大学校级项目(2015-JYB-JSMS021)

作者(Author): 曾鸿莲;刘振丽;宋志前;王淳;董运茁;宁张弛;赵思宇;舒一崧;何丹;王梦蕾;甘嘉荷;刘元艳;
ZENG Hong-lian;LIU Zhen-li;SONG Zhi-qian;WANG Chun;DONG Yun-zhuo;NING Zhang-chi;ZHAO Si-yu;SHU Yi-song;HE Dan;WANG Meng-lei;GAN Jia-he;LIU Yuan-yan;School of Chinese Materia Medica,Beijing University of Chinese Medicine;Institution of Basic Theory,China Academy of Chinese Medical Sciences;

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