邓炜;郑民强;张静;黄聪;张雅莉;
DENG Wei1*,ZHENG Minqiang1,ZHANG Jing1,HUANG Cong1,ZHANG Yali2(1.Department of Pharmacology,Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China; 2.Radioimmunoassay Room of First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang 550001,China)

• 1:贵阳中医学院药理教研室
• 2:贵阳中医学院第一附属医院

摘要(Abstract)：

目的:比较2种黔产淫羊藿总黄酮(TFE)对CTX小鼠免疫功能的影响。方法:采用CTX造免疫低下小鼠模型,同时给药10 d,以体重、脾重、脾指数,血清溶血素浓度,血清TNF-α及IL-2含量为指标,观察2种TFE增强免疫作用,并比较其差异。结果:单次ip CTX 80 mg.kg-1,使正常小鼠的脾指数、血清溶血素水平、IL-2和TNF-α含量明显下降;ig 2种TFE1.3,0.65 g.kg-1 10 d,可使体重、脾重、睥指数上升(P<0.01),下降的溶血素值回升(P<0.01),以及下降的IL-2和TNF-α含量显著升高(P<0.01)。结论:2种黔产淫羊藿TFE对CTX小鼠的非特异免疫和特异免疫功能都具有明显增强作用;但2种黔产淫羊藿茎叶TFE增强免疫作用差异不明显。提示TFE中除了淫羊藿苷(ICA)外,尚有其他增强免疫的有效成分。
Objective:Comparative study on the immunologic functions of total flavones of Epimedium(TFE) in two species in Guizhou on CTX mice.Method:To set up a hypoimmunity mouse model by CTX and give TFE in 10 days,the enhancement of immunologic functions of TFE of two species were observed with the indice of the weight of body and spleen,the spleen-index,the serum level of hemolysin,TNF-α and IL-2.Result:The spleen-index,the level of hemolysin,TNF-α and IL-2 of the mice decreased apparently(P<0.01)by single ip in CTX 80 mg·kg-1.The weight of body and spleen,the spleen-index of the mice increased,the decreased serum level of hemolysin TNF-α and IL-2 increased significantly(P<0.01),by TFE of two species of 1.3,0.65 g·kg-1 from the mice ig.Conclusion:The TFE of two species of Epimedium in Guizhou have an obvious immune enhancement effect on the non-specific immunity and specific immunity of CTX model mice.But the immune enhancement effects of the TFE from the stem and leaves of two species of Epimedium contrast insignificantly.This indicates that in addition to ICA,there must be other bioactive components that can enhance immunity.

关键词(KeyWords)： 黔岭淫羊藿;粗毛淫羊藿;淫羊藿总黄酮;增强免疫;比较研究
Epimedium leptorrhizum;Epimedium acuminatum;total flavones of Epimedium;immunologic functions

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅基金项目[黔科合J字(2006)2087号]

作者(Author): 邓炜;郑民强;张静;黄聪;张雅莉;
DENG Wei1*,ZHENG Minqiang1,ZHANG Jing1,HUANG Cong1,ZHANG Yali2(1.Department of Pharmacology,Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China; 2.Radioimmunoassay Room of First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang 550001,China)

Email:

DOI:

参考文献(References)：

• [1]李娌,王学美.淫羊藿苷药理作用研究进展[J].中国中药杂志,2008,33(23):2727.
• [2]徐文芬,何顺志,黄敏,等.高效液相色谱法测定贵州产淫羊藿药材不同药用部位中淫羊藿苷的含量[J].中国实验方剂学杂志,2007,13(5):1.
• [3]宋剑,王超,李知遥,等.淫羊蕾总黄酮抗老年痴呆化学成分研究[J].中国现代中药,2009,11(8):23.
• [4]陈奇.中药药理研究方法学(第2版)[M].北京:人民卫生出版社,2006:752.
• [5]杨静玉,于庆海,李爽,等.淫羊藿总黄酮对免疫功能低下小鼠的免疫增强作用[J].沈阳药科大学学报,1998,15(2):94.
• [6]葛林阜,董政军,姜国胜,等.淫羊藿甙对小鼠免疫调节作用的研究[J].中华实用医学,2001,3(3):25.