中国中药杂志

2008, (18) 2052-2055

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NIR和HPLC指纹图谱在熟地黄饮片质量稳定性考察中的对比研究
Comparisons between NIR and HPLC fingerprint on quality stability of Shudihuang decoction pieces

雷敬卫;白雁;樊克锋;
LEI Jing-wei1,2,BAI Yan2,FAN Ke-feng3 (1.Nanjing university of Traditional Chinese medicme,Nanjing 210046,China;2.Henan university of Traditional Chinese medicme,Zhengzhou 450008,China;3.Zhengzhou Colloge of Animal Husbandry Engineering,Zhengzhou 450011,China)

摘要(Abstract):

目的:建立近红外光谱法(NIR)考察熟地黄饮片质量稳定性的方法。方法:以NIR光谱聚类分析法对不同种类的熟地黄样品进行了聚类鉴别,并与HPLC指纹图谱方法进行了对比研究。结果:NIR光谱聚类分析结果反映了熟地黄饮片间的相似程度,与HPLC指纹图谱实验结果比较结果准确、分析过程简便。结论:NIR技术应用于熟地黄饮片质量的稳定性考察是可行和有效的,具有独特的优点。
Objective: To establish a new method for evaluating quality stability of Shudihuang decoction pieces with near-infrared spectrum.Method: Clustering analysis was used to distinguish different Shudihuang samples.And comparisons between NIR and HPLC fringerprint.Result: Results of clustering reflected the similar degree among these samples.Compared with HPLC fingerprint,NIR analytical process fast and convenient with exact results.Conclusion: NIR is a feasible and effective approach to examine the quality stability of Shudihuang decoction pieces with unique virtues.

关键词(KeyWords): 熟地黄;NIR;HPLC指纹图谱;聚类分析
Shudihuang;NIR;HPLC fingerprint;clustering analysis

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基金项目(Foundation): 2006年国家教育部科技研究重点资助项目(206087)

作者(Author): 雷敬卫;白雁;樊克锋;
LEI Jing-wei1,2,BAI Yan2,FAN Ke-feng3 (1.Nanjing university of Traditional Chinese medicme,Nanjing 210046,China;2.Henan university of Traditional Chinese medicme,Zhengzhou 450008,China;3.Zhengzhou Colloge of Animal Husbandry Engineering,Zhengzhou 450011,China)

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