中国中药杂志

2018, v.43(05) 938-944

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基于psbA-trnH序列对穿龙薯蓣及同属物种的鉴别研究
Identification of Dioscorea nipponica and its sects based on psbA-trnH barcode

赵容;邵飞;尹海波;康廷国;
ZHAO Rong;SHAO Fei;YIN Hai-bo;KANG Ting-guo;Liaoning University of Traditional Chinese Medicine;

摘要(Abstract):

利用psbA-trnH序列建立一种能快速精准,将穿龙薯蓣区别于其他同属物种的方法。该实验收集全国17个不同产区,共144份穿龙薯蓣样本,从GenBank上下载的23份薯蓣属其他植物的psbA-trnH序列,使用DNAMAN 8.0软件对测序后所得到的峰图进行拼接和校对,使用MEGA 6.0软件对序列进行分析比对。结果表明:穿龙薯蓣与毛胶薯蓣亲缘关系最近,与盾叶薯蓣亲缘关系最远,使用psbA-trnH序列作为DNA条形码能有效区分穿龙薯蓣与薯蓣属中的其他植物。
A rapid and accurate method was established by psbA-trnH sequence to distinguish Dioscorea nipponica from other species belonging to the same genus. In this study,we collected 144 samples of D. nipponica from 17 different producing areas in China,the psbA-trnH sequenced of 23 nucleotide sequences were downloaded from Gen Bank,the sequences from Dioscorea genus. DNAMAN 8. 0 software was used to show splicing,MEGA 6. 0 software was applied for sequence analysis and comparison. The results showed that the genetic relationship between D. nipponica and D. subcalva was the closest,and the genetic relationship with D. zingiberensis was the furthest. It is indicated that the psbA-trnH sequence as a DNA barcode can effectively distinguish between D. nipponica and other plants in the genus of Dioscorea.

关键词(KeyWords): psbA-trnH序列;穿龙薯蓣;薯蓣属
psbA-trnH sequences;Dioscorea nipponica;Dioscorea

Abstract:

Keywords:

基金项目(Foundation): 中国医药行业科研项目(201407002);; 辽宁省自然科学基金项目(2014020045);; 中央本级重大增减支项目(2060302-1603-09)

作者(Author): 赵容;邵飞;尹海波;康廷国;
ZHAO Rong;SHAO Fei;YIN Hai-bo;KANG Ting-guo;Liaoning University of Traditional Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180109.007

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