金艳;姚俊娜;格小光;张志杰;李娆娆;冯学锋;石金敏;
JIN Yan;YAO Jun-na;GE Xiao-guang;ZHANG Zhi-jie;LI Rao-rao;FENG Xue-feng;SHI Jin-min;State Key Laboratory Breeding Base of Dao-di Herbs,National Resources Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Pharmaceutical Sciences,Zhengzhou University;

• 1:中国中医科学院中药资源中心道地药材国家重点实验室培育基地
• 2:中国中医科学院中药研究所
• 3:郑州大学药学院

摘要(Abstract)：

基于药材性状和组织显微鉴别方法,对芫花及其混淆品河朔荛花的茎、叶进行了对比研究;并对芫花根进行了研究。结果表明芫花条药材或饮片主要为茎枝,节处有明显对生的叶柄痕和枝痕,一般未观察到叶或花序梗,折断面皮部有细密银白色絮状纤维。混淆品为带有茎枝、叶和花序梗的段,花序梗可见呈穗状花序的花梗痕。在显微组织方面,芫花的茎横切面韧皮部有单个散在的粗大的木化韧皮纤维,壁极厚;其叶下表皮附有单细胞非腺毛。河朔荛花茎横切面韧皮部未见粗大的木化韧皮纤维;叶表皮细胞外壁有厚角质层,下表皮无非腺毛。此结果可用于芫花条药材与饮片鉴别及质量标准制定。芫花根的组织和横切面观察可用于该品种根类药材的鉴别。
The purpose of this article is to identify Daphne genkwa and its adulterants,Wikstroemia chamaedaphne,according to the morphological and microstructure characteristics of their stem and foliage. The root of D. genkwa was studied simultaneously. The results indicated that the crude drug and processed pieces of Genkwa Ramulus were mainly composed of stems and branches where obvious opposite petiole scars and branch marks were able to be seen on their nodes. Otherwise,foliage or peduncles generally couldn' t be found. Moreover,the fine silver flocculent fibers could be observed in the bark of fracture surface. The adulterants were the plant segments which were composed of stems,foliage and peduncles with spikelet-pedicel scars. There existed microstructures differences between Genkwa Ramulus and its adulterants. In the former,single thick lignified phloem fibers were interspersed in the stem phloem of the transverse section with very thick wall and unicellular non-glandular hairs could be observed on the lower epidermis of foliage. Nevertheless,in the latter,there was no thick lignified phloem fibers in cross section of stem phloem,the outer wall of epidermal cells of foliage hadthick cuticles and no non-glandular hairs in lower epidermis of foliage. The results can be used for the identification and the quality standard of the crude drug and processed pieces of D. genkwa. The characteristics of the microstructures and the transverse section can be used to identify the radix D. genkwa.

关键词(KeyWords)： 芫花条;芫花;河朔荛花;芫花根;性状鉴别;显微鉴别
Genkwa Ramulus;Daphne genkwa;Wikstroemia chamaedaphne;Genkwa Radix(roots of Daphne genkwa);morphological dentification;microscopical identification

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2014ZX09304307001-002-010,2012ZX09202101-005);; 北京市自然科学基金项目(7112097);; 国家自然科学基金项目(815032338);; 国家中医药行业科技专项(201507002-2,201507002-3)

作者(Author): 金艳;姚俊娜;格小光;张志杰;李娆娆;冯学锋;石金敏;
JIN Yan;YAO Jun-na;GE Xiao-guang;ZHANG Zhi-jie;LI Rao-rao;FENG Xue-feng;SHI Jin-min;State Key Laboratory Breeding Base of Dao-di Herbs,National Resources Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Pharmaceutical Sciences,Zhengzhou University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20171030.012

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