周惠芬;艾进超;万海同;何昱;张宇燕;赵涛;付巍;杨洁红;
ZHOU Hui-fen;AI Jin-chao;WAN Hai-tong;HE Yu;ZHANG Yu-yan;ZHAO Tao;FU Wei;YANG Jie-hong;Zhejiang Chinese Medicine University;Shandong Buchang Pharmaceutical Company Limited;

• 1:浙江中医药大学
• 2:山东步长制药有限公司

摘要(Abstract)：

为研究参芎注射液对脑缺血再灌注老龄大鼠炎症损伤的影响。将84只SD大鼠随机分为假手术组、模型组、尼莫通组、参芎注射液高、中、低剂量组。连续腹腔注射给药3 d,采用线栓法建立大鼠脑缺血再灌注模型,再灌注24 h,神经功能评分,取脑TTC染色,ELISA法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的含量,荧光实时定量PCR(RTPCR)对海马区IL-1β,TNF-α,ICAM-1和MMP-9 m RNA的表达量进行检测。结果,参芎注射液可使脑梗死体积减少,明显改善神经功能,参芎注射液能减少IL-1β,TNF-α,ICAM-1和MMP-9 m RNA的表达量,并减少IL-1β和TNF-α的含量。表明参芎注射液对大鼠缺血再灌注损伤具有明显的保护作用,其机制可能与抑制炎症因子表达有关。
To investigate the effect of Shenxiong injection on the inflammation injury of ischemia-reperfusion injury senile rats. Totally 84 Sprague-Dawley( SD) rats were randomly divided into six groups: the sham operation group,the model group,the Nimodipine group and the Shenxiong injection( low,middle,and high dosage) groups. The rat cerebral ischemia-reperfusion model was established through intraperitoneal injection for 3 d and middle cerebral artery occlusion( MCAO). After the reperfusion for 24 h,efforts were made to give neurological score,collect brains for TTC staining,detect tumor necrosis factor-α( TNF-α) and interleukin-1β( IL-1β)content in serum by enzyme-linked immunosorbent assay( ELISA) method and measure IL-1β,ICAM-1 and MMP-9 m RNA expressions in hippocampal area by Real-time PCR( RT-PCR). According to the results,Shenxiong injection could decrease the cerebral infarction volume,greatly improved the neurological function and reduce IL-1β,TNF-α,ICAM-1 and MMP-9 m RNA expressions and IL-1β and TNF-α contents. In conclusion,Shenxiong injection shows the significant protective effect on ischemia-reperfusion injury in rats. Its mechanism may be related to the inhibition of inflammatory factor expression.

关键词(KeyWords)： 参芎注射液;脑缺血再灌注损伤;TNF-α;IL-1β;ICAM-1;MMP-9
Shenxiong injection;cerebral ischemia-reperfusion injury;TNF-α;IL-1β;ICAM-1;MMP-9

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81274176,81202636);; 浙江省卫生高层次创新人才培养工程项目

作者(Author): 周惠芬;艾进超;万海同;何昱;张宇燕;赵涛;付巍;杨洁红;
ZHOU Hui-fen;AI Jin-chao;WAN Hai-tong;HE Yu;ZHANG Yu-yan;ZHAO Tao;FU Wei;YANG Jie-hong;Zhejiang Chinese Medicine University;Shandong Buchang Pharmaceutical Company Limited;

Email:

DOI:

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