中国中药杂志

2011, v.36(11) 1478-1482

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白头翁属药用植物的HPLC指纹特征与化学分类研究
Study on HPLC fingerprint characteristics and chemotaxonomy of Pulsatilla medicinal plants

李海燕;李宏博;郝宁;许永男;朴钟云;
LI Haiyan1,LI Hongbo1,HAO Ning1,XU Yongnan2,PIAO Zhongyun1(1.College of Horticulture,Shenyang Agricultural university,Shenyang 110866,China;2.SK Chemicals Lab of Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

摘要(Abstract):

目的:建立白头翁属5种药用植物皂苷类成分的HPLC指纹特征图谱,为这5种药用植物的鉴定及亲缘关系评价提供依据。方法:采用HPLC测定白头翁属药用植物的指纹图谱,依据获得的色谱数据对各样品进行分析与鉴定。结果:建立了白头翁属5种药用植物中皂苷类成分的HPLC指纹图谱,这5种药用植物均显示了其共性和各自的指纹特征;确立10个色谱峰为共有峰。运用系统聚类将14份样品聚成4类:第Ⅰ,Ⅱ,Ⅳ类分别为朝鲜白头翁,蒙古白头翁和白头翁;第Ⅲ类由兴安白头翁、细叶白头翁组成。朝鲜白头翁与兴安白头翁,蒙古白头翁与细叶白头翁之间的化学亲缘关系较远。结论:聚类结果与传统分类结果有较好的一致性。HPLC化学图谱可用于白头翁属植物的分类和鉴别研究,并为白头翁属植物指纹图谱研究和质量控制提供重要参考。
Objective:To establish HPLC characteristic fingerprints of the saponins in Pulsatilla medicinal plants,and provide the basis for authentication and classification of Pulsatilla species.Method:The HPLC profiles were determined at 35℃ on a Symmetry C18 column(4.6 mm×250 mm,5 μm) eluted with water(A) and acetonitrile(B) as mobile phases in a linear gradient elution with the flowrate of 0.5 mL·min-1.The elution program was as follows:0-8 min,90%A to 77%A,8-25 min,changed to 71% A,25-40 min,to 60% A,40-50 min,to 50%A,50-75 min,to 10% A,75-80 min,to 0% A.The detection wavelength was set at 210 nm.Result:The different species of Pulsatilla showed different HPLC fingerprints,but with 10 common peaks.A cluster analysis of 14 accessions indicated that they were divided into four groups:all accessions from P.koreana were classified into group Ⅰ,P.ambigua in group Ⅱ,P.dahurica and P.turczaninovii in group Ⅲ,and P.chinensis in group Ⅳ,respectively.The significant differences between P.koreana and P.dahurica,and between P.turczaninovii and P.ambigua were observed.Conclusion:The results obtained were in agreement with the traditional taxonomic study.The method was rapid and precise,not only can be used to classify and authenticate Pulsatilla species,but also provides important references for HPLC fingerprints and quality control of Pulsatilla medicinal plants.

关键词(KeyWords): 白头翁属;HPLC指纹图谱;聚类分析
Pulsatilla adans;HPLC fingerprint;cluster analysis

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Keywords:

基金项目(Foundation): 中韩合作项目(SK2007005)

作者(Author): 李海燕;李宏博;郝宁;许永男;朴钟云;
LI Haiyan1,LI Hongbo1,HAO Ning1,XU Yongnan2,PIAO Zhongyun1(1.College of Horticulture,Shenyang Agricultural university,Shenyang 110866,China;2.SK Chemicals Lab of Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

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