蒋超;罗宇琴;袁媛;黄璐琦;金艳;赵玉洋;
JIANG Chao;LUO Yu-qing;YUAN Yuan;HUANG Lu-qi;JIN Yan;ZHAO Yu-yang;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

• 1:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

为建立一种能同时鉴别人参、三七、西洋参及其掺杂品的方法。通过分析人参属ITS,18S和matK序列,寻找特异性SNP位点设计引物,建立多重位点特异性PCR法,并对不同来源的人参、三七、西洋参样品进行扩增,根据特异性条带大小进行鉴别。在退火温度为60℃,循环数为35时,人参、三七和西洋参分别出现约250,500,1000 bp的特异性条带。该方法对于人参、三七、西洋参相互掺杂的混合样品,人参中掺杂三七或西洋参以及三七中掺杂西洋参或人参的检出限均为0.5%,对西洋参中掺杂人参的检出限为0.5%,掺杂三七的检出限为1%。表明建立的多重位点特异性PCR可用于人参、西洋参、三七的掺杂鉴定。
To achieve a molecular method to identify Panax ginseng,P.notoginseng,P.quinquefolius and their admixture.The ITS,18 S and mat K sequences of Panax genus were analyzed to develop species-specific SNP marker.Three pairs of species-specific primers were designed to establish a multiplex allele-specific polymerase chain reaction(MAS-PCR) and the samples from different regionwere tested.The results showed that when the annealing temperature was 60 ℃ and the cycle number was 35,approximately 250,500,1 000 bp specific band were obtained from P.ginseng,P.notoginseng and P.quinquefolius obtain,respectively.This method could also be used to authentificate admixture samples and could detect 0.5% percent of P.notoginseng or P.quinquefolius adulterated in P.ginseng,or 0.5% percent of P.ginseng or P.quinquefolius adulterated in P.notoginseng.The detect limit of P.ginseng in P.quinquefolius was 0.5% and P.notoginseng in P.quinquefolius was 1%.This results showed that the present method could be used as a promise method to identify Panax ginseng,P.notoginseng,P.quinquefoliusand their admixture.

关键词(KeyWords)： 人参;西洋参;三七;多重位点特异性PCR;掺伪;分子鉴定
Panax ginseng;Panax notoginseng;Panax quinquefolius;multiplex allele-specific polymerase chain reaction;admixture;molecular identification

Abstract:

Keywords:

基金项目(Foundation): 中医药行业专项(2014007003)

作者(Author): 蒋超;罗宇琴;袁媛;黄璐琦;金艳;赵玉洋;
JIANG Chao;LUO Yu-qing;YUAN Yuan;HUANG Lu-qi;JIN Yan;ZHAO Yu-yang;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20170222.001

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