中国中药杂志

2014, v.39(17) 3311-3315

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中药通关藤红外指纹图谱研究
FTIR fingerprint spectrograms of traditional Chinese medicine Marsdenia tenacissima

李超;杨生超;郭巧生;郑开颜;王平理;徐祥增;肖雪峰;
LI Chao;YANG Sheng-chao;GUO Qiao-sheng;ZHENG Kai-yan;WANG Ping-li;XU Xiang-zeng;XIAO Xue-feng;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;Yunnan Agriculture University;Yunnan Xintong Botanic Pharmaceutical Co.,Ltd.;

摘要(Abstract):

采用傅里叶变换红外光谱(FTIR)技术对4省份16产区的48份通关藤样品进行分析,建立通关藤药材FTIR指纹图谱。通过对该指纹图谱的解析表征、全谱对比、共有特征峰对比、主成分分析和聚类分析等方法,建立快速鉴别不同产地通关藤的方法。结果表明,不同产地通关藤样品的FTIR图谱存在一定的差异性,运用适当的化学计量学方法可实现该药材的产地鉴别;指纹图谱解析、相关系数分析、主成分分析和聚类分析等结果能从不同层面对通关藤产地进行正确的分类,结果相互验证和补充。本研究为通关藤药材的产地鉴别和质量控制提供了一种新的技术和方法。
In this paper,fourier transform infrared spectroscopy fingerprint analysis of Marsdenia tenacissima samples was used to develop a reliable method of tracing the geographical origins.Forty-eight samples from four provinces of China were analyzed by FTIR.We analyzed and characterized the fingerprints in both the full spectrum peaks and characteristic peaks,then the principal component analysis and the cluster analysis were carried out.The results of fingerprint analysis,correlation analysis,principal component analysis and cluster analysis can identify the geographic origins correctly,which verified and supplemented each other;the identification results and the actual location showed a high degree of consistency,namely the lower the space distance,the greater the similarity of different samples.These results revealed the obvious superiority and practical value in comparison to the more tedious and time-consuming wet chemistry method normally used.Using appropriate metrology methods can trace die geographical source correctly.The M.tenacissima materials from the region of Maguan should be considered as genuine medicinal materials taking into account the good quality.

关键词(KeyWords): 通关藤;FTIR指纹图谱;相关系数;主成分分析;聚类分析
Marsdenia tenacissima;FTIR fingerprint spectrograms;coefficient of correlation;principal component analysis;cluster analysis

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作者(Author): 李超;杨生超;郭巧生;郑开颜;王平理;徐祥增;肖雪峰;
LI Chao;YANG Sheng-chao;GUO Qiao-sheng;ZHENG Kai-yan;WANG Ping-li;XU Xiang-zeng;XIAO Xue-feng;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;Yunnan Agriculture University;Yunnan Xintong Botanic Pharmaceutical Co.,Ltd.;

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