中国中药杂志

2012, v.37(08) 1100-1106

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

基于居群DNA条形码的药用植物黄芩的产地鉴别研究
Locality identification of Chinese medicinal plant Scutellaria baicalensis(Lamiaceae) population-level DNA barcoding

张彬;袁庆军;黄璐琦;刘晓光;李晓明;林淑芳;陈美兰;格小光;
ZHANG Bin,YUAN Qingjun,HUANG Luqi,LIU Xiaoguang,LI Xiaoming,LIN Shufang,CHEN Meilan,GE Xiaoguang(Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

摘要(Abstract):

目的:利用居群DNA条形码阐明不同产地(居群)黄芩的遗传分化程度,对其进行产地鉴别。方法:应用进化速率快和母系遗传的叶绿体DNA基因间序列,基于分子谱系地理学理论筛选出居群间遗传分化明显的片段,构建单倍型网状进化树,根据单倍型在网状进化树中的位置、出现的频率和地理分布范围的大小,划分出不同层次的居群DNA条形码,以用于地理分布范围宽窄不同的产地鉴别。结果:本实验筛选出3个叶绿体DNA基因间序列atpB-rbcL,trnL-trnF和psbA-trnH对17个黄芩野生居群进行分子谱系地理学分析,得到13个多态位点,共20个单倍型,其中3个分布最广、频率最高的单倍型可用于较广地域的产地鉴别,称为区域居群DNA条形码;3个分布次之、只存在于2~3个邻近居群的单倍型可用于较窄地域的产地鉴别,称为地区居群DNA条形码;另有8个仅局限在某一个居群的单倍型可用于特定居群的鉴别,称为居群特有DNA条形码。结论:叶绿体基因间序列在居群间存在明显遗传分化,可用于地理分布范围宽窄不同的黄芩产地鉴别。
Scutellaria baicalensis is an important traditional Chinese medicine and Scutellaria flavonoids have received worldwide attention in recent years.It is the basis of controlling quality of S.baicalensis to develop a reliable genetic marker system used to identify locality of origin.Because of the characteristics of maternal inherited and high-rate of evolution,the cpDNA intergenic spacer can effectively elucidate the degree of genetic variation in different areas of the same species(populations),which can be used as the population-level DNA barcoding to locality identify.In this study,we have used the molecular phylogeography analysis for the three cpDNA intergenic spacers atpB-rbcL,trnL-trnF and psbA-trnH of 17 wild populations from different localities,which reveals the 20 haplotypes,including 13 polymorphic sites and constitutes a shallow gene tree.The authers have divided the haplotypes of S.baicalensis into three grades of population-level DNA barcoding according to the frequence and geographic distribution: 3 highest-frequency haplotypes as area-population-level DNA barcoding,3 haplotypes were mainly shared by 2-3 adjacent populations as region-population-level DNA barcoding,and there are also 8 unique-population haplotypes as unique-population-level DNA barcoding.The result of this study reveals that population-level DNA barcoding is a reliable genetic marker used to locality identify of S.baicalensis.

关键词(KeyWords): 黄芩;居群DNA条形码;遗传分化;产地鉴别
Scutellaria baicalensis;population-level DNA barcoding;genetic variation;locality identify

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81130070,81072989);; 国家中医药管理局行业科研专项(201107009)

作者(Author): 张彬;袁庆军;黄璐琦;刘晓光;李晓明;林淑芳;陈美兰;格小光;
ZHANG Bin,YUAN Qingjun,HUANG Luqi,LIU Xiaoguang,LI Xiaoming,LIN Shufang,CHEN Meilan,GE Xiaoguang(Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享