中国中药杂志

2013, v.38(13) 2155-2158

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基于HPLC指纹图谱的不同产地升麻质量评价研究
Study on quality evaluation of Cimicifugae Rhizoma from different producing areas by HPLC fingerprint

沈保家;秦昆明;张星海;刘启迪;蔡皓;刘晓;蔡宝昌;
SHEN Bao-jia1,2,3,QIN Kun-ming1,2,3,ZHANG Xing-hai1,LIU Qi-di1,CAI Hao1,3,LIU Xiao1,2,CAI Bao-chang1,2,3(1.Engineering Research Center for Processing Standardization of Traditional Chinese Medicines under State Ministry of Education,Nanjing University of Chinese Medicine,Nanjing 210029,China; 2.Nanjing Haichang Chinese Medicine Group Co.,Ltd.,Nanjing 210061,China; 3.Key Laboratory for Processing Standardization of Traditional Chinese Medicines under State Administration of Traditional Chinese Me

摘要(Abstract):

建立不同产地升麻药材的指纹图谱。使用Kromasil色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%甲酸溶液,梯度洗脱,柱温35℃,流速1.0 mL.min-1,检测波长254 nm。提取12个色谱峰作为指纹图谱共有峰,采用相似度评价、聚类分析、主成分分析等方法,对所收集的21批样品进行系统比较与归类。结果确定了12个共有峰,将不同产地的样品分为3类。该法重复性好,简便可靠,可以为不同产地升麻的质量控制和评价提供依据。
To establish a fingerprint for Cimicifugae Rhizoma from different producing areas.Column kromasil(4.6 mm×250 mm,5 μm) was employed with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution.The flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm.Twenty chromatographic peaks were extracted as the common peaks of fingerprint,and 21 batches of samples were compared and classified with such methods as similarity evaluation,cluster analysis and principle component analysis.The results showed 12 common peaks and three categories of samples.The method was so highly reproducible,simple and reliable that it could provide basis for quality control and evaluation of Cimicifugae Rhizoma from different producing areas.

关键词(KeyWords): 升麻;指纹图谱
Cimicifugae Rhizoma;fingerprint

Abstract:

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基金项目(Foundation): 国家科技部火炬计划项目(2010GH020663);; 江苏省科技支撑计划-工业项目(BE2011012);; 江苏省科技成果转化项目(BA2010024);; 南京市科技公共服务平台建设项目(201105007);; 南京市科技发展计划项目(201007005)

作者(Author): 沈保家;秦昆明;张星海;刘启迪;蔡皓;刘晓;蔡宝昌;
SHEN Bao-jia1,2,3,QIN Kun-ming1,2,3,ZHANG Xing-hai1,LIU Qi-di1,CAI Hao1,3,LIU Xiao1,2,CAI Bao-chang1,2,3(1.Engineering Research Center for Processing Standardization of Traditional Chinese Medicines under State Ministry of Education,Nanjing University of Chinese Medicine,Nanjing 210029,China; 2.Nanjing Haichang Chinese Medicine Group Co.,Ltd.,Nanjing 210061,China; 3.Key Laboratory for Processing Standardization of Traditional Chinese Medicines under State Administration of Traditional Chinese Me

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