中国中药杂志

2006, (03) 209-212

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配伍对麻黄汤中甘草HPLC指纹图谱的影响
Research on the influence of compatibility on fingerprint of Glycyrrhiza uralensis in Mahuang decoctions by HPLC

吴昭晖;罗佳波;谭晓梅;
WU Zhao-hui,LUO Jia-bo,TAN Xiao-mei(Key laboratory of Research of Traditional Chinese Medicine and New Drug of Southern Medical University,Guangzhou 510515,China)

摘要(Abstract):

目的:建立麻黄汤各配伍煎液中甘草的HPLC指纹图谱,研究配伍对甘草指纹图谱的影响。方法:采用反相高效液相色谱法,选用Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-1.2%醋酸溶液(梯度洗脱),分析时间70 min,检测波长为254 nm。结果:该方法稳定、可靠、重复性好,可用于甘草药材和麻黄汤中甘草指纹图谱的测定。标定了麻黄汤中甘草指纹图谱8个共有指纹峰,考察了这8个共有峰峰面积在麻黄汤不同配伍的变化。结论:配伍对麻黄汤中甘草指纹图谱的影响显著,使甘草7个共有指纹峰的峰面积显著降低,6个共有指纹峰的峰面积比值显著升高。
Objective: To set up the HPLC fingerprint of Glycyrrhiza uralensis in decoctions prepared from various combinations of the recipe named Mahuang decoctions and study the influence of compatibility on fingerprint of G.uralensis in decoctions.Method: The RP-HPLC method was used,chromatography conditions were Zorbax SB-C_(18) column(4.6 mm×250 mm,5 μm),the acetonitrile-1.2% acetic acid(gradient elution) as mobile phase and detective wavelength at 254 nm.Result: The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of G.uralensis,it can be used to determine the fingerprint of G.uralensis in Mahuang decoctions.The result showed that 8 peaks were common in the fingerprint of G.uralensis in Mahauang decoctions.The area changes of 8 common peaks in decoctions prepared from various combinations of Mahuang decoctions were studied.Conclusion: The fingerprint of(G.uralensis) in Mahuang decoctions is markedly influenced by herba ephedrae and ramulus cinnamomi and semen armeniacae amarum.The areas of 7 common peaks were reduced obviously,while the relative areas of 6 common peaks were increased remarkably.

关键词(KeyWords): 麻黄汤;配伍;甘草;指纹图谱;HPLC
Mahuang decoctions;compatibility;Glycyrrhiza uralensis;fingerprint;HPLC

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金重点资助项目(30030150)

作者(Author): 吴昭晖;罗佳波;谭晓梅;
WU Zhao-hui,LUO Jia-bo,TAN Xiao-mei(Key laboratory of Research of Traditional Chinese Medicine and New Drug of Southern Medical University,Guangzhou 510515,China)

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