中国中药杂志

2019, v.44(02) 338-343

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丹参波长叠加指纹图谱及多指标成分测定研究
Study on wavelength overlapping HPLC fingerprint and multi-component content determination of Danshen

王涛;张慧;赵丹;陈杰;谢卓霖;戴维;叶坤浩;黄晶;陈岗福;
WANG Tao;ZHANG Hui;ZHAO Dan;CHEN Jie;XIE Zhuo-lin;DAI Wei;YE Kun-hao;HUANG Jing;CHEN Gang-fu;Institute of Chinese Medicinal Materials,Mianyang Academy of Agricultural Sciences;Comprehensive Experimental Station of Mianyang,Chinese Materia Medica of China Agriculture Research System;Biotechnology Center,Mianyang Academy of Agricultural Sciences;School of Life and Engineering,Southwest University of Science and Technology;Mianyang Dadi Maidong Industrial Technology Research Institute;Sichuan Daida

摘要(Abstract):

对丹参波长叠加指纹图谱和7种丹参指标成分同时定量进行研究。采用Agilent Eclipse Plus C_(18)(4. 6 mm×100 mm,3. 5μm)色谱柱,以0. 1%甲酸的水溶液(A)-乙腈(B)为流动相梯度洗脱(0~5 min,10%~20%B; 5~20 min,20%~30%B; 20~25 min,30%~50%B; 25~40 min,50%~65%B; 40~45 min,65%~80%B; 45~46 min,80%~10%B; 46~50 min,10%B),流速1mL·min~(-1),柱温30℃,检测波长250,280,310,340 nm。运用波长叠加技术建立指纹图谱,并选取主要成分建立多指标测定方法。波长叠加指纹图谱体现了丹参250,280,310,340 nm特征吸收波长的指纹信息,17个不同来源的丹参样品与指纹图谱之间具有良好的相似性,相似度在0. 828~0. 936。确定了15个共有峰,并指认了其中7个色谱峰,分别是迷迭香酸、丹酚酸B、丹酚酸A、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA,其方法学考察结果良好。建立了丹参波长叠加谱指纹图谱,该方法简便可靠、耐用性好,可用于丹参的质量控制。
This project was launched to and establish the wavelength overlapping HPLC fingerprint for Danshen and determine the contents of 7 component in Danshen. The chromatographic fingerprints were built by using Agilent Eclipse Plus C_(18)( 4. 6 mm×100 mm,3. 5 μm) as stationary phase and 0. 1% formic acid solution( A)-acetonitrile( B) as mobile phase with gradient elution( 0-5 min,10%-20% B; 5-20 min,20%-30% B; 20-25 min,30%-50% B; 25-40 min,50%-65% B; 40-45 min,65%-80% B; 45-46 min,80%-10% B; 46-50 min,10% B) at a flow rate of 1 mL·min~(-1). The column temperature was maintained at 30 ℃ and the detection wavelength was set at 250,280,310 and 340 nm. The technique of wavelength overlapping fingerprint was established and the contents of 7 indicative compounds have been determined in this method. The results of wavelength overlapping HPLC fingerprint showed all-around information of the fingerprints at 250,280,310 and 340 nm,and the similarity among 17 batches of Danshen was over 0. 828-0. 936. In wavelength overlapping HPLC fingerprint,15 common peaks were selected as the common peaks,and 7 contents of them were indentified as rosmarinic acid,salvianolic acid B,salvianolic acid A,phenolic acid,dihydrotanshinone Ⅰ,tanshinone Ⅰ and tanshinone ⅡA. The results of methodological study demonstrated that the method met the requirements of the determination. The method established in this study is simple,convenient and durable,which can provide a scientific basis for the quality control of Danshen.

关键词(KeyWords): 丹参;高效液相色谱;波长叠加;指纹图谱;多指标成分
Danshen;HPLC;wavelength overlapping;fingerprint;multi-component content

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项(CARS-21)

作者(Author): 王涛;张慧;赵丹;陈杰;谢卓霖;戴维;叶坤浩;黄晶;陈岗福;
WANG Tao;ZHANG Hui;ZHAO Dan;CHEN Jie;XIE Zhuo-lin;DAI Wei;YE Kun-hao;HUANG Jing;CHEN Gang-fu;Institute of Chinese Medicinal Materials,Mianyang Academy of Agricultural Sciences;Comprehensive Experimental Station of Mianyang,Chinese Materia Medica of China Agriculture Research System;Biotechnology Center,Mianyang Academy of Agricultural Sciences;School of Life and Engineering,Southwest University of Science and Technology;Mianyang Dadi Maidong Industrial Technology Research Institute;Sichuan Daida

Email:

DOI: 10.19540/j.cnki.cjcmm.20181009.011

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