赵英永;崔秀明;戴云;苗华;
ZHAO Ying-yong~(1,2),CUI Xiu-ming~(1,3),DAI Yun~(2),MIAO Hua~(4)(1.Wenshan Prefecture Sanqi Science and Technology Research Institute,Wenshan 663000,China;2.Yunnan Nationalities University,Kunming 650031,China;3.Wenshan Sanqi Science & Technology Innovation Center,Wenshan 663000,China;4.Nanyang Normal University,Nanyang 473061,China)

• 1:文山州三七科学技术研究所
• 2:文山州三七科学技术研究所
• 3:云南民族大学化学与生物技术学院
• 4:南阳师范学院 云南文山663000

摘要(Abstract)：

目的:采用HPLC建立草乌药材的指纹图谱。方法:Kromasil C18色谱柱(4.6 mm×250 mm,5μm);乙腈-醋酸铵缓冲溶液(2.5‰冰醋酸-浓氨水,pH 10.5)(60∶40)为流动相;流速1.0 mL.min-1;检测波长240 nm;柱温30℃。结果:精密度和重复性试验中各共有峰相对峰面积的RSD均小于2%,所得的色谱图各色谱峰分离较好,基线平整,符合指纹图谱有关规定。结论:本方法快速、简便、准确、重复性好,结果可靠,可为草乌药材的质量评价提供有效手段。
Objective: To establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.Method: The chromatographic separation was performed on a Kromasil C_(18)(4.6 mm×250 mm,5 μm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer(2.5‰ acetic acid-ammonia pH 10.5)(60∶40).The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL·min~(-1).Result: The RSD of precision and repeatability was less than 2%.Under the selected chromatographic conditions,good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.Conclusion: The method was simple,accurate and repeatable.It can be used for the quality control of Radix Aconiti Kusnezoffii.

关键词(KeyWords)： 草乌;HPLC;指纹图谱;质量控制
Radix Aconiti Kusnezoffii;fingerprint;HPLC;quality control

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵英永;崔秀明;戴云;苗华;
ZHAO Ying-yong~(1,2),CUI Xiu-ming~(1,3),DAI Yun~(2),MIAO Hua~(4)(1.Wenshan Prefecture Sanqi Science and Technology Research Institute,Wenshan 663000,China;2.Yunnan Nationalities University,Kunming 650031,China;3.Wenshan Sanqi Science & Technology Innovation Center,Wenshan 663000,China;4.Nanyang Normal University,Nanyang 473061,China)

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参考文献(References)：

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• [2]李家实.中药鉴定学.上海:上海科学技术出版社,1996:78.
• [3]屠鹏飞.中药指纹图谱制度的方法学讨论.国际色谱指纹图谱评价中药质量研讨会论文集.广州.2001.
• [4]屠鹏飞.中药材及中药注射剂指纹图谱研究的体会.中药指纹图谱学术研讨会论文集.上海.2000.