色谱指纹图谱在制南星饮片质量标准研究的应用Study on applying chromatographic fingerprints to establishing quality criterion of processed tuber of Pinellia pedatisecta
赫炎,张启伟,张永欣,孙洁,王祝举
HE Yan, ZHANG Qi-wei, ZHANG Yong-xin, SUN Jie, WANG Zhu-ju(Institute of Chinese Materia Medica, China Academy of Traditional Chinese Med icine, Beijing 100700, China)[
摘要(Abstract):
目的 :通过虎掌南星饮片研究 ,探讨指纹图谱在中药质量标准建立的定量应用。方法 :HPLC指纹图谱检测和化学计量分析 (windowpreprocessing ,principalcomponentanalysis)。结果 :建立虎掌南星饮片指纹图谱的定量表述及定量范围 ;并以此表征饮片产地和批之间物质基础变异。结论 :用windowpreprocessing ,principalcomponentanalysis分析和标识饮片HPLC指纹图谱 ,并以此作为饮片质量控制指标是可行的。
Objective: To explore the utility of princ ip al component analysis (PCA) in conjunction with the window preprocessing scheme on chromatographic data for quality control. Method: Chrom atographic fingerprints of processed tuber of Pinellia pedatisecta were dete r mined by HPLC, and the Window Preprocessing and PCA were used for data processin g. Result: The quantitative differences among different gr owing areas and different process batches were found with this method. Conclusion: The method can be used in quality control for monitorin g between-batch products of traditional Chinese pharmaceutical process. [
关键词(KeyWords):
主成分分析;虎掌南星;质量控制
principal component analysis; Pinellia pe datisecta; quality control
基金项目(Foundation):
作者(Author):
赫炎,张启伟,张永欣,孙洁,王祝举
HE Yan, ZHANG Qi-wei, ZHANG Yong-xin, SUN Jie, WANG Zhu-ju(Institute of Chinese Materia Medica, China Academy of Traditional Chinese Med icine, Beijing 100700, China)[
Email:
DOI:
参考文献(References):
- [1] 程翼宇,陈闽军,吴永江.化学指纹图谱的相似性测度及其评价方法.化学学报,2002,60(11):2017.
- [2] 梁逸曾,谢培山.指纹图谱的化学模式识别分析.世界科学技术.中药现代化,2002,4(5):47.
- [3] 中国药典.一部.2000.43.
- [4] WelshWJ,LinW ,TersigniSH ,etal.Pharmaceuticalfingerprint ing:evaluationofneuralnetworksandchemometrictechniquesfordis tinguishingamongsame productmanufacturers.AnalChem,1996,68:3473.
- [5] CollantesER ,DutaR ,WelshWJ.PreprocessingofHPLCtraceim puritypatternsbywaveletpacketsforpharmaceuticalfingerprintingus ingartificialneuralnetworks.AnalChem,1997,69:1392.
- 赫炎
- 张启伟
- 张永欣
- 孙洁
- 王祝举
HE Yan - ZHANG Qi-wei
- ZHANG Yong-xin
- SUN Jie
- WANG Zhu-ju(Institute of Chinese Materia Medica
- China Academy of Traditional Chinese Med icine
- Beijing 100700
- China)[
- 赫炎
- 张启伟
- 张永欣
- 孙洁
- 王祝举
HE Yan - ZHANG Qi-wei
- ZHANG Yong-xin
- SUN Jie
- WANG Zhu-ju(Institute of Chinese Materia Medica
- China Academy of Traditional Chinese Med icine
- Beijing 100700
- China)[