中国中药杂志

2019, v.44(15) 3335-3342

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陈皮降脂药效与黄酮类成分的相关性研究
Correlation between lipid-lowering efficacy and components of Pericarpium Citri Reticulatae

俞静静;苏洁;颜美秋;楼招欢;吕圭源;
YU Jing-jing;SU Jie;YAN Mei-qiu;LOU Zhao-huan;LYU Gui-yuan;College of Pharmaceutical Science,Zhejiang Chinese Medical University;

摘要(Abstract):

基于药效-成分相关性分析,筛选陈皮最佳降脂部位,并确定可能的药效成分,为陈皮可能的新药开发提供基础。该研究以TC,LDL-c为指标,采用脂肪乳剂致高脂血症大鼠模型筛选陈皮最佳降脂部位;采用HPLC-ESI-MS技术,通过色谱峰的保留时间,m/z,UV光谱对陈皮不同溶剂提取物共有成分进行归属和鉴别分析;采用HPLC-DAD测定黄酮类成分(芸香柚皮苷、橙皮苷、香风草苷、川陈皮素、橘皮素、3,5,6,7,8,3',4'-七甲氧基黄酮)含量;采用SPSS双变量相关分析方法,对降脂药效与成分进行相关性分析。结果显示陈皮不同溶剂提取物均可显著降低脂肪乳剂致高血脂大鼠血清TC,LDL-c含量,以95%乙醇部位、乙酸乙酯部位效果最佳。陈皮5个不同溶剂提取物有19个共有峰,归属鉴别了17个黄酮类成分,其中多甲氧基黄酮类有10个,其他黄酮类有7个。按生药量计,二氢黄酮含量:正丁醇部位>95%乙醇部位>水提部位>乙酸乙酯部位>石油醚部位;多甲氧基黄酮含量:正丁醇部位≈95%乙醇部位≈乙酸乙酯部位>石油醚部位>水提部位。相关性分析表明血脂降低与多甲氧基黄酮量呈正相关,提示多甲氧基黄酮可能为陈皮降脂有效部位。
Based on pharmacodynamics-component correlation analysis,the best effective part of hyperlipidemia of Pericarpium Citri Reticulatae( PCR) was screened out to confirm the possible constituents with the lipid regulating effect,in order to provide a basis for the development of new drugs. Hyperlipidemia rats induced by fat emulsion were used to screen out the best hypolipidemic parts of PCR with TC,LDL-c as the index. HPLC-ESI-MS were used to analyze common constituents of the different solvent extracts from PCR. The constituents were classified and identified based on the retention time,m/z and UV spectra. And the HPLC-DAD were used to determine the contents of flavonoids( narirutin,hesperidin,didymin,nobiletin,tangeretin,3,5,6,7,8,3',4'-heptemthoxyflavone).Correlation analysis was conducted on the constituents and efficacy with the method of SPSS ANOVA bivariate correlation. Five extracts could significantly decrease the content of TC,LDL-c of hyperlipemia rats induced by fat emulsion,and the best effective part were95% ethanol and ethyl acetate. There were 19 common peaks in five different solvent extracts from PCR,and 17 flavonoids were identified and classified,including 10 polymethoxyflavonoids and 7 other flavonoids. According to the raw material quantity,the order of content of flavonones arranged from high to low: n-butyl alcohol part> 95% ethanol part> water part> ethyl acetate part> petroleum ether part; and the order of PMFs arranged from high to low: n-butyl alcohol part≈95% ethanol part≈ethyl acetate part > petroleum ether part > water part. The decreased percentage of TC,LDL-c was positively correlated with 10 common PMFs constituents,which suggested that PMFs may be the effective components for reducing blood lipid.

关键词(KeyWords): 陈皮;多甲氧基黄酮;二氢黄酮;高血脂;Pearson相关性分析
Pericarpium Citri Reticultae;polymethoxyflavonoids(PMFs);flavonones;hyperlipidemia;Pearson correlation analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81803819);; 浙江省自然科学基金项目(LQ17H280004,LQ18H280003,LQ17H280005);; 浙江省重点实验室项目(2012E10002)

作者(Author): 俞静静;苏洁;颜美秋;楼招欢;吕圭源;
YU Jing-jing;SU Jie;YAN Mei-qiu;LOU Zhao-huan;LYU Gui-yuan;College of Pharmaceutical Science,Zhejiang Chinese Medical University;

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