知母及含知母成药中菝葜皂甙元的薄层扫描法测定TLC-Densitometric Determination of Sarsasapogenin in Anemarrhena asphodeloides Bge
孙文基,沙振方,杨春光
Sun Wenji, Sha Zhenfang Yang Chunguang(Shaanxi Provincial Institute for Drug Control, Xian)
摘要(Abstract)：
用薄层扫描法测定了知母药材及含知母中成药中菝葜皂甙元的含量。苯-丙酮(9:1)为溶剂,香草醛-硫酸显色,测定波长445nm,参比波长600nm。
The sarsasapogenin content in Anemarrhena asphodeloides and its preparations was assayed by TLC-densitometric method. The developing solvent used was benzene-acetone (9: 1). The chromatographic plate was sprayed with a mixture of 8% vanillin in alcoholic solution-70% H_2SO_4 solutoin(0.5: 5). This plate was scanned using 445nm as wavelength λ_s and 600nm as wavelength λ_R.
关键词(KeyWords)：
菝葜皂甙元;知母;薄层扫描
sarsasapogenin; TLC-densitometry; Anemarrhena asphodeloides
基金项目(Foundation):
作者(Author):
孙文基,沙振方,杨春光
Sun Wenji, Sha Zhenfang Yang Chunguang(Shaanxi Provincial Institute for Drug Control, Xian)