中国中药杂志

2012, v.37(07) 966-968

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

茯苓UPLC特征指纹图谱
UPLC characteristic chromatographic profile of Poria

张琦;王振中;萧伟;张靓琦;毕开顺;贾英;
ZHANG Qi1,WANG Zhenzhong2,XIAO Wei2,ZHANG Liangqi1,BI Kaishun3,JIA Ying *(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China; 2.Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China; 3.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

摘要(Abstract):

建立茯苓的UPLC特征指纹图谱分析方法,为快速评价茯苓的质量,完善茯苓的质量控制方法提供依据。采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相乙腈-0.05%磷酸溶液,梯度洗脱,检测波长243 nm。建立了茯苓UPLC特征指纹图谱共有模式,标定了20个共有峰,指认了其中7个共有峰,15批茯苓的相似度在0.787~0.974。该方法快速,可用于评价茯苓的质量。
To establish a UPLC characteristic chromatographic profile analysis method to quickly assess Poria quality and provide basis fro controlling Poria quality.The UPLC characteristic chromatographic profiles of fifteen batches of Poria were determined by ACQUITY UPLC,with HSS T3 Column(2.1 mm×100 mm,1.8 μm) eluted with the mobile phases of water containing 0.05% phosphoric acid and acetonitrile in gradient mode.The detection wavelength was set at 243 nm.The common mode of the UPLC characteristic chromatographic profile was set up.There were 20 common peaks,seven of which were identified,and the similar degrees of the fifteen samples to the common mode were between 0.787 and 0.974.The method was so time-saving that it can be used for the quality control of Poria.

关键词(KeyWords): 茯苓;特征指纹图谱;UPLC
Poria;characteristic chromatographic profile;UPLC

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003)

作者(Author): 张琦;王振中;萧伟;张靓琦;毕开顺;贾英;
ZHANG Qi1,WANG Zhenzhong2,XIAO Wei2,ZHANG Liangqi1,BI Kaishun3,JIA Ying *(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China; 2.Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China; 3.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享