姜新刚;孟作环;贾继明;
JIANG Xingang,MENG Zuohuan,JIA Jiming(Shijiazhuang Yiling Pharmaceutical Co.,Ltd.,Shijiazhuang 050035,China)

• 1:石家庄以岭药业股份有限公司

摘要(Abstract)：

目的:采用高效毛细管电泳法建立广西菲牛蛭药材的指纹图谱。方法:石英毛细管柱(75μm×56 cm)作为分离通道,以25 mmol.L-1Na2HPO4-120 mmol.L-1Tris-16 mmol.L-1SDS(1 mol.L-1NaOH调pH 12.0)为缓冲液,运行电压17kV,柱温25℃,检测波长254 nm,进样参数3.4 kPa×6 s,运行时间27 min。结果:建立了广西菲牛蛭药材的高效毛细管电泳指纹图谱,确定了共有峰为13个,10批药材指纹图谱与对照指纹图谱相似度均大于0.98。结论:该方法具有良好的精密度、稳定性和重复性,为有效控制该药材质量提供了新方法。
Objective:To establish fingerprints for Poecilobdella manillensis from Guangxi province using the high performance capillary electrophoresis(HPCE) method.Method:Electrophoresis was performed on a fused silica capillary column(75 μm×56 cm),with 25 mmol·L-1 Na2HPO4-120 mmol·L-1 Tris-16 mmol·L-1 SDS(adjusted to pH 12.0 with 1 mol·L-1 NaOH) as the running buffer.The applied voltage was 17 kV,the temperature was 25 ℃ and the detection wavelength was 254 nm.The sample's hydrodynamic injection was 3.4 kPa×6 s and the duration was 27 min.Result:HPCE fingerprint was established with 13 common peaks.The similarity between fingerprints of P.manillensis in 10 batches and control fingerprints was more than 0.98.Conclusion:The method is so precise,reproducible and stable that it could be used as a new means for the quality control of P.manillensis.

关键词(KeyWords)： 高效毛细管电泳;指纹图谱;菲牛蛭
HPCE;fingerprints;Poecilobdella manillensis

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX0901-020)

作者(Author): 姜新刚;孟作环;贾继明;
JIANG Xingang,MENG Zuohuan,JIA Jiming(Shijiazhuang Yiling Pharmaceutical Co.,Ltd.,Shijiazhuang 050035,China)

Email:

DOI:

参考文献(References)：

• [1]杨潼.中国动物志环节动物门蛭纲[M].北京:科学出版社,1996:120.
• [2]周维官,周维海,覃国森.菲牛蛭的人工养殖方式试验[J].广西科学,2008,15(3):317.
• [3]欧兴长,张秋海,丁家欣,等.四种水蛭抗凝血酶作用研究[J].天然产物研究与开发,1996,8(2):54.
• [4]吴志军,于立华.菲牛蛭及其制品的抗血栓、溶血栓作用[J].中成药,2007,29(4):591.
• [5]李文,廖福龙,殷晓杰,等.七种水蛭抗血小板聚集与抗凝血研究[J].中药药理与临床,1997,13(5):32.
• [6]苗艳丽,方福永,宋文东.中药菲牛蛭化学成分的分析[J].中成药,2007,29(8):1248.
• [7]黄爱民.广西菲牛蛭中菲牛蛭素的分离纯化及体内外抗凝血实验[D].南宁:广西医科大学,2005.
• [8]Verena Steiner,Rene Knecht,Markus Gruetter,et al.Isolationand purification of novel hirudins from the leech Hirudinaria ma-nillensis by high performance liquid chromatography[J].J Chroma-togr B,1990,530:273.
• [9]中国药典.一部[S].2010:77.