中国中药杂志

2004, (11)

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双参通冠方对急性心肌缺血再灌注损伤时TNF-α,ICAM-1的影响
Effects of Shuangshen tongguan(SSTG) on TNF-α, ICAM-1 during myocardial ischemia-reperfusion injury

韩笑,刘建勋,马晓斌,王杨慧
HAN Xiao, LIU Jian-xun, MA Xiao-bin, WANG Yang-hui (Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Be ijing 100091,China) [

摘要(Abstract):

目的 :观察双参通冠方 (SSTG)对急性心肌缺血再灌注损伤动物模型心肌梗死范围及血清中肿瘤坏死因子 (TNF α)、细胞间黏附分子 1(ICAM 1)含量的影响。方法 :冠状动脉结扎 /放松法复制大鼠心肌缺血再灌注损伤模型 ,分为正常组 (假手术组 )、模型组、SSTG高、低剂量组 ,N BT染色法测量心肌梗死范围 ,双抗体夹心ABC ELISA法测定各组血清中TNF α ,ICAM 1的含量。结果 :缺血再灌注损伤模型组心肌梗死面积及梗死区重量明显异常 ,血清中TNF α ,ICAM 1含量增高 (P <0 .0 5 )。SSTG处理后心肌梗死面积缩小、梗死重量减轻、梗死 /心室 (心脏 )百分比降低 ,血清中TNF α ,ICAM 1含量下调 (P <0 .0 5 )。结论 :受缺血再灌注刺激时血清中TNF α,ICAM 1含量增加 ,双参通冠方可能通过抑制TNF α ,ICAM 1的过量分泌而保护受损心肌 ,表现为心肌梗死面积减小、梗死区重量减轻及梗死 /心室 (心脏 )百分比降低
Objective: To observe the effects of SSTG on in fa rction size and tumor necrosis factor-α(TNF-α), intercellular adhesion mo lecular-1 (ICAM-1)levels in serum during reperfusion injury of acute myocar dial ischemia. Method: Anterior descending branch of coronary artery was ligated and released to create myocardial ischemia-reperfusion injur y . The size and weight of infarction area and the contents of TNF-α, ICAM-1 in serum were assayed by N-BT staining and ELISA respectively. Result : The size and weight of infarct area and the contents of TNF-α, ICA M-1 in serum were significantly increased compared with the normal group and we re obviously decreased after being treated with SSTG. Conclusion: Ischemia-reperfusion stimulated the secretion of TNF-α and ICAM-1, whic h play an important role in ischemia-reperfusion injury. SSTG might protect myo cardium from ischemia-reperfusion injury by suppressing over-secretion of TNF -α and ICAM-1 and reducing the size and weight of infarction area. [

关键词(KeyWords): 缺血再灌注损伤;肿瘤坏死因子;细胞间黏附分子-1
Ischemia-reperfusion injury; tumor necrosis fa ctor-α; intercellular adhesion molecular-1

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基金项目(Foundation): 国家自然科学基金资助项目 (3 0 17114 9)

作者(Author): 韩笑,刘建勋,马晓斌,王杨慧
HAN Xiao, LIU Jian-xun, MA Xiao-bin, WANG Yang-hui (Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Be ijing 100091,China) [

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