中国中药杂志

2010, v.35(06) 686-689

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丹参及其近缘种花粉活力与柱头可授性研究
Pollen viability and stigma receptivity of Salvia miltiorrhiza and its relative

钟国成;张利;杨瑞武;丁春邦;
ZHONG Guocheng,ZHANG Li*,YANG Ruiwu,DING Chunbang(College of Biology and Science,Sichuan Agricultural University,Ya′an 625014,China)

摘要(Abstract):

目的:研究8份不同产地丹参和4种丹参近缘植物有性生殖特性,为其人工杂交育种积累资料。方法:用TTC法测定花粉的活力,用联苯胺-过氧化氢法测定柱头可授性。结果:河南丹参(包括栽培丹参和野生丹参)的花粉活力随散粉时间的延长逐渐降低;在开花当天花粉活力最高;其他产地的丹参及丹参近缘植物花粉活力随散粉时间的延长变化情况为先升高,再降低;在开花后23 d花粉活力最高。开花期间陕西丹参花粉活力变异最大(RSD 71.3%);河南野生丹参花粉活力变异最小(RSD 12.4%)。花粉活力平均值最高的是河南野生丹参(72.3%),最低的是云南鼠尾草(38.8%)。丹参及其近缘植物柱头在花冠刚开放时便具有可授性;短唇鼠尾草柱头可授性较强时期在花开后23 d花粉活力最高。开花期间陕西丹参花粉活力变异最大(RSD 71.3%);河南野生丹参花粉活力变异最小(RSD 12.4%)。花粉活力平均值最高的是河南野生丹参(72.3%),最低的是云南鼠尾草(38.8%)。丹参及其近缘植物柱头在花冠刚开放时便具有可授性;短唇鼠尾草柱头可授性较强时期在花开后24 d;其余材料开花期间可授性强弱变化相对较小。花粉及柱头寿命可维持34 d;其余材料开花期间可授性强弱变化相对较小。花粉及柱头寿命可维持35 d。结论:丹参及其近缘植物人工授粉的最佳时期是开花后25 d。结论:丹参及其近缘植物人工授粉的最佳时期是开花后23 d。
Objective: To provide the basal data for artificial cross breeding of Chinese herb Salvia miltiorrhiza from 7 provinces in China and its 4 relatives.Method: The pollen viability was evaluated by TTC(2,3,5-triphenylte trazolium chloride) test and the stigma receptivity was evaluated by benzidine-H2O2 method.Result: The pollen viability of S.miltiorrhiza from 6 provinces in China and its 4 relatives deceased during time of pollen shedding.Their highest pollen viability was in 2 or 3 days after blooming.But the pollen viability of S.miltiorrhiza(wild and culture) from Hean province in China declined with time after blooming.The most obvious variation of the pollen viability was in S.miltiorrhiza from Shanxi province(RSD 71.3%) and the least was in wild S.miltiorrhiza from Henan province(RSD 12.4%).The highest average pollen viability was wild S.miltiorrhiza(72.3%) from Henan province while the lowest was S.yunnanensis(38.8%).The stigmas of all the accessions had receptivity when blooming.The stigma receptivity of S.brevilabra was strong in 2 to 4 days after blooming,while the others had less change after blooming.The life span of pollen grains and stigmas could be maintained from 3 to 5 days.Conclusion: The optimum artificial pollination time of S.miltiorrhiza and its relatives was 2 to 3 days after blooming.

关键词(KeyWords): 丹参;鼠尾草属;花粉活力;柱头可授性
Salvia miltiorrhiza;Salvia;pollen viability;stigma receptivity

Abstract:

Keywords:

基金项目(Foundation): 四川省青年科技基金项目(08ZQ026-034);; 四川省科技厅科技支撑项目(2008FZ0148,2008SZ0074);; 四川省科技厅应用基础项目(07jy029-023)

作者(Authors): 钟国成;张利;杨瑞武;丁春邦;
ZHONG Guocheng,ZHANG Li*,YANG Ruiwu,DING Chunbang(College of Biology and Science,Sichuan Agricultural University,Ya′an 625014,China)

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