中国中药杂志

2015, v.40(12) 2403-2407

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龙牙楤木总皂苷对缺血/再灌注心肌细胞收缩功能和钙瞬变的影响
Effect of aralosides to contraction function and calcium transient of ischemia / reperfusion myocardial cells

张妙笛;孙桂波;徐惠波;王敏;孙晓波;
ZHANG Miao-di;SUN Gui-bo;XU Hui-bo;WANG Min;SUN Xiao-bo;Research Center on Life Sciences and Environmental Sciences,Harbin University of Commerce;Pharmacology and Toxicology Center,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences;Academy of Chinese Medical Sciences of Jilin Province;

摘要(Abstract):

为探讨龙牙楤木总皂苷(AS)对缺血/再灌注(I/R)诱导大鼠心肌细胞损伤的保护作用,采用乳酸钠灌注液灌注成年大鼠的心室肌细胞模拟缺血,含钙台式液模拟再灌注,细胞收缩与离子浓度同步测定系统同步检测AS对单个I/R细胞收缩和钙瞬变的影响。发现AS使再灌注后心肌细胞静息态肌小节长度、收缩幅度、收缩/舒张速度以及钙瞬变幅度、速度增大(P<0.05,P<0.01),细胞达到舒张时最大长度的90.0%的时间、细胞收缩达峰值的时间、稳态钙静息值、收缩期[Ca2+]i上升到最高的50.0%的时间、胞内钙瞬变衰减率减少(P<0.05,P<0.01)。因此,推测AS改善了缺血再灌注后细胞收缩与钙稳态的损伤。
To discuss the protective effect of aralosides(AS) on I/R-induced rat myocardial injury. The adult rat ventricular myocyte ischemia model was established through perfusion with sodium lactate perfusate and reperfusion with Ca2 +-containing Tyrode's solution simulation. The cell contraction and ion concentration synchronization determination system was applied to detect the effect of AS on single I / R cell contraction and Ca2 +transients. According to the findings,AS could increase resting sarcomere length,contraction amplitude, ± d L / dtmax,calcium transient amplitude and speed of post-reperfusion myocardial cells(P < 0. 05,P < 0. 01),and decrease in time for achieving 90. 0% of maximum relaxation,time for achieving peak value,resting calcium ratio,contraction period[Ca2 +]i,time for achieving 50. 0% of maximum relaxation and attenuation rate of intracellular calcium transient(P < 0. 05,P <0. 01). Therefore,it is suggested that AS improved the post-reperfusion cell contraction and injury of calcium homeostasis.

关键词(KeyWords): 龙牙楤木总皂苷;心肌细胞;缺血再灌注;细胞收缩与离子浓度同步测定系统
aralosides;myocyte;ischemia/reperfusion;cell contraction and ion concentration synchronization determination system

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基金项目(Foundation): 国家自然科学基金项目(81173589,81473380);; 北京市自然科学基金项目(7142108);; 高等学校博士学科点专项科研基金(20121106110033)

作者(Author): 张妙笛;孙桂波;徐惠波;王敏;孙晓波;
ZHANG Miao-di;SUN Gui-bo;XU Hui-bo;WANG Min;SUN Xiao-bo;Research Center on Life Sciences and Environmental Sciences,Harbin University of Commerce;Pharmacology and Toxicology Center,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences;Academy of Chinese Medical Sciences of Jilin Province;

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