中国中药杂志

2017, v.42(17) 3398-3402

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补体抑制活性物质鱼腥草总多糖中内毒素的去除
Removal of lipopolysaccharides from anti-complementary crude polysaccharides of Houttuynia Herba

张娟娟;张庆琳;卢燕;陈道峰;
ZHANG Juan-juan;ZHANG Qing-lin;LU Yan;CHEN Dao-feng;School of Pharmacy,Fudan University;

摘要(Abstract):

为建立补体抑制活性鱼腥草总多糖中内毒素的去除方法,将琼脂糖疏水色谱及聚丙烯酰胺凝胶过滤色谱分别与多粘菌素亲和吸附色谱联用纯化鱼腥草多糖。以显色基质比色法检测纯化前后内毒素的含量变化,细胞溶血法检测纯化前后体外抗补体活性变化。结果发现,疏水色谱与亲和吸附色谱的联用对内毒素的清除效果优于凝胶过滤色谱与亲和吸附色谱的联用,其清除率达42.85%。内毒素清除前后,抗补体活性没有明显变化。表明疏水色谱与亲和吸附色谱联用可用于清除鱼腥草总多糖中的内毒素且不影响其抗补体活性。
An Affi-Prep Polymyxin column was combined with a Phenyl Sepharose column and a Sephacryl S-300 column,respectively,to remove the lipopolysaccharides( LPS) in the anti-complementary crude polysaccharides of Houttuynia Herba. The contents of LPS in the polysaccharides were determined by chromogenic tachypleus amebocyte lysate( TAL) method during the procedure of purifying. The anti-complementary activities of the polysaccharides were also compared before and after the removal of LPS. Less remanent LPS was detected after purified using Penyl Sepharose combined with polymyxin column,with the clearance rate of 42. 85%. All the columns had no effect on the anti-complementary activity of the polysaccharides. Penyl Sepharose combined with polymyxin column would be sound for LPS removal of the anti-complementary polysaccharides without reducing their bioactivity.

关键词(KeyWords): 鱼腥草总多糖;内毒素;抗补体;疏水色谱;亲和吸附色谱
crude polysaccharides of Herba Houttuynia;LPS;anti-complementary;Penyl Sepharose;polymyxin

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2012ZX09301001-003);; 国家自然科学基金项目(81330089,30801508)

作者(Author): 张娟娟;张庆琳;卢燕;陈道峰;
ZHANG Juan-juan;ZHANG Qing-lin;LU Yan;CHEN Dao-feng;School of Pharmacy,Fudan University;

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DOI: 10.19540/j.cnki.cjcmm.20170728.011

参考文献(References):

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