赫炎;赵慧东;唐力英;王祝举;张启伟;
HE Yan1,ZHAO hui-dong2,TANG Li-ying2,WANG Zhu-ju2,ZHANG Qi-wei2(1.Institute of Basic Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

• 1:中国中医科学院中医临床基础医学研究所
• 2:中国中医科学院中药研究所
• 3:中国中医科学院中药研究所
• 4:中国中医科学院中药研究所

摘要(Abstract)：

目的:探讨如何应用HPLC指纹图谱的计量技术对白芍炒制过程及白芍饮片进行质量控制。方法:中试生产研制白芍饮片,HPLC,PFA(principal factor analysis)。结果:获得了较稳定的白芍饮片HPLC图谱;在不同产地和不同饮片品种图谱的10个特征峰中,通过各个峰之间的联带变化在不同饮片中的分布规律,运用PFA的信息处理技术,抽取出能反映对HPLC谱中峰群有影响的2个公共因子,其累计贡献率为64.8%,根据各峰对因子的载荷和communality筛选出的7个峰可作为主要反映图谱形状变化的目标峰群。结论:HPLC指纹图谱和PFA可用于白芍的炒制工艺过程和饮片的质量控制。
Objective:To explore the utility of Principal Factor Analysis(PFA) in chromatographic data for quality control.Method: Chromatographic fingerprints of processed root pieces of Paeonia lactiflora were determined by HPLC,the PFA was used for data processing.Result: The quantitative differences among different growing areas and different processing batches were found with the method.Conclusion: The method could be used in quality control for monitoring between-batch products of traditional Chinese pharmaceutical process.

关键词(KeyWords)： 白芍;PFA;HPLC;色谱指纹图谱
Principal Factor Analysis;Paeonia lactiflora;chromatographic fingerprints

Abstract:

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作者(Author): 赫炎;赵慧东;唐力英;王祝举;张启伟;
HE Yan1,ZHAO hui-dong2,TANG Li-ying2,WANG Zhu-ju2,ZHANG Qi-wei2(1.Institute of Basic Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

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参考文献(References)：

• [1]中国药典[S].一部.2000:68.
• [2]赫炎,张启伟,张永欣,等.色谱指纹图谱在制南星饮片质量标准研究的应用[J].中国中药杂志,2004,29(9):874.pharm aceutical process.