中国中药杂志

2011, v.36(06) 715-717

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牡丹皮药材UPLC特征指纹图谱研究
UPLC characteristic chromatographic profile of Moutan Cortex

范旭航;王振中;李清;马天成;毕开顺;贾英;
FAN Xuhang1,WANG Zhenzhong2,LI Qing3,MA Tiancheng1,BI Kaishun3,JIA Ying1*(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China; 2.Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China; 3.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

摘要(Abstract):

目的:建立牡丹皮药材的超高效液相色谱特征指纹图谱分析方法。方法:采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm);流动相乙腈-0.05%磷酸水,梯度洗脱,检测波长254 nm。结果:建立了牡丹皮UPLC特征指纹图谱共有模式,标定了20个共有峰,并指认了10个主要色谱峰,15批牡丹皮药材的相似度为0.973~0.998。结论:本方法快速、高效,可用于牡丹皮药材的质量评价。
Objective: To develop an UPLC method of determining the characteristic chromatographic profiles of Moutan Cortex for quality control of the medicine.Method: The UPLC characteristic chromatographic profiles of fifteen batches of Moutan Cortex were determined on an HSS T3 column(2.1 mm×100 mm,1.8 μm) eluted with the mobile phase consisted of water containing 0.05% phosphoric acid and acetonitrile in gradient mode and the detection wavelength was set at 254 nm.Result: The common mode of the UPLC characteristic chromatographic profile was set up under the established condition.There were 20 common peaks in the characteristic chromatographic profile of fifteen samples,ten of which were identified,and the similar degrees of the fifteen batches to the common mode were between 0.973-0.998.Conclusion: The method was fast and accurate.The characteristic chromatographic profile of Moutan Cortex with high specificity can be used to control the quality of Moutan Cortex.

关键词(KeyWords): 牡丹皮;特征指纹图谱;UPLC
Moutan Cortex;characteristic chromatographic profile;UPLC

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09504-004)

作者(Author): 范旭航;王振中;李清;马天成;毕开顺;贾英;
FAN Xuhang1,WANG Zhenzhong2,LI Qing3,MA Tiancheng1,BI Kaishun3,JIA Ying1*(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China; 2.Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China; 3.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

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