中国中药杂志

2010, v.35(06) 759-762

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梅花鹿茸胶原酶解物对维甲酸致大鼠骨质疏松防治作用的实验研究
Prevention and therapeutic effects of sika deer velvet collagen hydrolysate on osteoporosis in rats by retinoic acid

李银清;赵雨;孙晓迪;曲晓波;
LI Yinqing,ZHAO Yu*,SUN Xiaodi,QU Xiaobo(Changchun University of Traditional Chinese Medicine,Changchun 130117,China)

摘要(Abstract):

目的:观察梅花鹿茸胶原酶解物(CSDV)对维甲酸诱导大鼠骨质疏松症的影响。方法:大白鼠50只,随机分为5组:对照组,模型组和CSDV高、中、低剂量组(0.4,0.2,0.1 g.kg-1.d-1)。通过灌胃给予维甲酸70 mg.kg-1.d-1,连续14d,建立大鼠骨质疏松模型。造模同时灌胃给予鹿茸胶原酶解物治疗30 d后,处死动物。取血分离血清,测定血清钙、磷、碱性磷酸酶含量;取左侧股骨称重,计算骨重系数,做骨生物力学强度测定;取右侧股骨,做骨组织形态计量测定。结果:与模型组比较,CSDV能明显改善对维甲酸诱导大鼠的骨密度降低,血清碱性磷酸酶升高,钙、磷含量降低;改善骨病理形态发生的明显改变;骨小梁个数减少、平均骨小梁宽度变窄、平均骨小梁间距变大、骨体积降低、平均骨皮质厚度变薄;改善骨生物力学强度指标;股最大载荷降低、最大挠度变短、骨应力减小、骨应变量降低等骨质疏松改变。但上述功能随鹿茸胶原酶解物给药剂量变化而不同。结论:鹿茸胶原酶解物中剂量组能使骨量增加,骨组织显微结构趋于正常,骨生物力学强度增加,对大鼠骨质疏松有防治作用。
The objective was to evaluate the preventive and therapeutic effects of the collagen hydrolysate extracted from Sika deer velvet(CSDV) on osteoporosis rats induced by retinoicacid.Histomorphometric indices and serum biochemical parameters were measured in osteoporosis rats treated with/without antler collagen and in sham-operated rats.Our results were as follows: compared with the osteoporosis group,significant elevation in the levels of bone mineral density(BMD),Ca,P and static histomorphometric indexes and biomechanical properties,but reduction in the level of serum alkaline phosphatase(ALP) were observed in antler collagen-treated groups.However,the above function with the collagenase solution velvet material varied with the different doses.In conclusion,the extracted collagen is found to play a role in the prevention and treatment of osteoporosis rats by retinoic acid.

关键词(KeyWords): 鹿茸胶原;骨质疏松;酶解
antler collagen;osteoporosis;hydrolysate

Abstract:

Keywords:

基金项目(Foundation): 国家科技部“十一五”计划支持项目(2006BAI06A05-01,2006BAI06A20-01)

作者(Authors): 李银清;赵雨;孙晓迪;曲晓波;
LI Yinqing,ZHAO Yu*,SUN Xiaodi,QU Xiaobo(Changchun University of Traditional Chinese Medicine,Changchun 130117,China)

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