中国中药杂志

2015, v.40(12) 2318-2324

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指纹图谱结合化学计量学用于香橼品种鉴别的可行性分析
Identification of two varieties of Citri Fructus by fingerprint and chemometrics

苏静华;张超;孙磊;顾炳仁;马双成;
SU Jing-hua;ZHANG Chao;SUN Lei;GU Bing-ren;MA Shuang-cheng;Suzhou Institute for Food and Drug Control;National Institutes for Food and Drug Control;Xinjiang Institute for Food and Drug Control;

摘要(Abstract):

该文拟探讨利用指纹图谱结合化学计量学手段进行香橼品种鉴别的可行性。共有23批香橼样本,涵盖《中国药典》收载的香圆和枸橼品种,采用高效液相色谱法采集色谱图。对部分特征峰进行初步鉴别,建立共有模式,用相似度评价、二维聚类分析、主成分分析(PCA)和偏最小二乘判别分析(PLS-DA)进行考察。结果表明由25个特征峰组成的共有模式可以有效将香圆和枸橼区分,稳定性好,并且模式识别也初步验证了结果的可靠性。进一步利用绝对峰面积(APA)进行相对定量,体现出品种之间的量化差异,对今后质控所选择合适的指标提供了一定基础。
Citri Fructus identification by fingerprint and chemometrics was investigated in this paper. Twenty-three Citri Fructus samples were collected which referred to two varieties as Cirtus wilsonii and C. medica recorded in Chinese Pharmacopoeia. HPLC chromatograms were obtained. The components were partly identified by reference substances,and then common pattern was established for chemometrics analysis. Similarity analysis,principal component analysis( PCA),partial least squares-discriminant analysis(PLS-DA) and hierarchical cluster analysis heatmap were applied. The results indicated that C. wilsonii and C. medica could be ideally classified with common pattern contained twenty-five characteristic peaks. Besides,preliminary pattern recognition had verified the chemometrics analytical results. Absolute peak area( APA) was used for relevant quantitative analysis,results showed the differences between two varieties and it was valuable for further quality control as selection of characteristic components.

关键词(KeyWords): 指纹图谱;化学计量学;香橼;高效液相色谱
fingerprint;chemometrics;Citri Fructus;HPLC

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作者(Author): 苏静华;张超;孙磊;顾炳仁;马双成;
SU Jing-hua;ZHANG Chao;SUN Lei;GU Bing-ren;MA Shuang-cheng;Suzhou Institute for Food and Drug Control;National Institutes for Food and Drug Control;Xinjiang Institute for Food and Drug Control;

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