中国中药杂志

2020, v.45(20) 4949-4956

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基于指纹图谱和多成分含量测定的穿山龙药材质量评价研究
Quality control of Dioscoreae Nipponicae Rhizoma based on fingerprint and quantitative analysis

杨贵雅;常雅晴;薛紫鲸;宋军娜;张丹;郑玉光;郭龙;
YANG Gui-ya;CHANG Ya-qing;XUE Zi-jing;SONG Jun-na;ZHANG Dan;ZHENG Yu-guang;GUO Long;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy,Hebei University of Chinese Medicine;

摘要(Abstract):

穿山龙为薯蓣科植物穿龙薯蓣Dioscorea nipponica的干燥根茎。根据生长栽培方式的不同,穿山龙药材可分为野生品和栽培品2种。该文采用高效液相色谱串联蒸发光散射检测器法对12批穿山龙野生品和10批穿山龙栽培品中甾体皂苷类成分进行了分析,建立了穿山龙药材的化学指纹图谱,共标定6个共有峰,穿山龙样品相似度均在0.950以上,所建立的穿山龙化学指纹图谱专属性良好,可用于穿山龙药材的质量评价。在此基础上,对穿山龙野生品和栽培品中的原薯蓣皂苷、原纤细薯蓣皂苷、甲基薯蓣皂苷、伪原薯蓣皂苷、薯蓣皂苷、纤细薯蓣皂苷6种甾体皂苷类成分进行了含量测定,进一步结合主成分分析和偏最小二乘法判别分析等化学计量学方法对穿山龙野生品和栽培品进行区分与比较。结果表明穿山龙野生品和栽培品所含甾体皂苷类成分种类无差异,而各甾体皂苷成分含量有明显差异,其中原薯蓣皂苷和原纤细薯蓣皂苷是二者的差异化合物,可作为鉴别和区分穿山龙野生品和栽培品的指标。通过以上研究,为穿山龙药材的质量控制、野生品和栽培品的鉴别与区分提供了分析方法和数据支撑。
Dioscoreae Nipponicae Rhizoma, the dried rhizoma of Dioscorea nipponica, has been widely used in traditional Chinese medicines. According to the different of the growth and cultivation patterns, Dioscoreae Nipponicae Rhizoma can be divided into two species, the wild Dioscoreae Nipponicae Rhizoma and the cultivated Dioscoreae Nipponicae Rhizoma. In this paper, an accurate and reliable fingerprint of Dioscoreae Nipponicae Rhizoma was established based on HPLC coupled with evaporative light scattering detector(ELSD). A total of 6 common peaks were marked, and the similarity of the Dioscoreae Nipponicae Rhizoma samples was above 0.950. The results indicated that the established fingerprint could be used for quality evaluation of Dioscoreae Nipponicae Rhizoma. Moreover, an HPLC coupled with ELSD method was developed for simultaneous quantitative analysis of six steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, dioscin and gracillin in wild Dioscoreae Nipponicae Rhizoma and cultivated Dioscoreae Nipponicae Rhizoma samples. Furthermore, chemometrics analysis such as principal component analysis and partial least squares discriminant analysis were performed to compare and discriminate wild Dioscoreae Nipponicae Rhizoma and cultivated Dioscoreae Nipponicae Rhizoma samples based on the quantitative data. The results indicated that the contents of steroidal saponins were notably different between the wild and cultivated Dioscoreae Nipponicae Rhizoma, and protodioscin and protogracillin were significant to effectively discriminate the wild and cultivated Dioscoreae Nipponicae Rhizoma samples, and these two compounds could be recognized as chemical markers. In conclusion, this present study might provide useful data and acceptable analysis method for identification and quality evaluation of Dioscoreae Nipponicae Rhizoma.

关键词(KeyWords): 穿山龙;指纹图谱;含量测定;质量控制;化学计量学
Dioscoreae Nipponicae Rhizoma;fingerprint;quantitative analysis;quality control;chemometrics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81803697);; 河北省自然科学基金项目(H2018423032,H2017423048);; 河北省普通高等学校青年拔尖人才计划项目(BJ2019006);; 河北省中医药管理局科研计划项目(2018108);; 河北省第二期现代农业产业技术体系创新团队项目(HBCT2018060205);; 第四次全国中药资源普查项目(Z135080000022)

作者(Author): 杨贵雅;常雅晴;薛紫鲸;宋军娜;张丹;郑玉光;郭龙;
YANG Gui-ya;CHANG Ya-qing;XUE Zi-jing;SONG Jun-na;ZHANG Dan;ZHENG Yu-guang;GUO Long;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy,Hebei University of Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.20200615.201

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