杨雪晶;熊爱珍;杨莉;王长虹;王峥涛;
YANG Xuejing1,XIONG Aizhen2,YANG Li2,WANG Changhong2,WANG Zhengtao1,2,3* (1.Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China; 2.The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Shanghai Key Laboratory for Compound Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,Chin

• 1:中国药科大学生药学研究室
• 2:上海中医药大学中药研究所中药标准化教育部重点实验室
• 3:上海中医药大学中药研究所上海市复方中药重点实验室

摘要(Abstract)：

目的:建立千里光Senecio scandens和欧洲千里光S.scandens非生物碱部位的HPLC特征指纹图谱分析方法,并对二者进行比较研究。方法:采用HPLC-UV方法对7批千里光药材和6批欧洲千里光药材的指纹图谱进行了比较,并分别以绿原酸、金丝桃苷为对照品,对指纹峰进行了半定量分析;采用Shiseido Capcell Pak MGⅡC18(4.6 mm×250 mm,5μm)色谱柱,流动相乙腈-0.2%乙酸,梯度洗脱。结果:2种千里光属植物HPLC非生物碱部位的特征指纹图谱差异显著,分别在千里光和欧洲千里光指纹谱中检出11个共有峰,HPLC-DAD及HPLC-MS分析结果表明上述共有峰主要为有机酸类(包括绿原酸类和原儿茶酸类化合物)和黄酮类化合物。半定量分析的结果表明千里光中有机酸和黄酮类化合物的含量较高,其平均含量分别为欧洲千里光中的2.39,15.56倍。结论:所建立HPLC方法稳定可靠,同时适用于千里光属植物千里光和欧洲千里光的指纹图谱研究,可用于千里光药材的质量评价。
Objective: To develop an HPLC method for fingerprint study of both Senecio scandens and S.scandens.Method: Fingerprints of the two Senecio herbs were compared.And the concentrations of main peaks in them were semi-quantified as chlorogenic acid or hyperoside.Chromatography was performed on a Shiseido Capcell Pak MG Ⅱ C18(4.6 mm×250 mm,5 μm) column using a gradient elution of acetonitrile-water containing 0.2% acetic acid.And detection wavelength was 360 nm.Result: Significant difference was found in the fingerprint of the two herbs.Eleven peaks were picked out for the evaluation of S.scandens and S.scandens,respectively.They were identified to be either organic acid compounds or flavones by HPLC-UV and LC-ESI-MS analysis.And semi-quantification of them showed the concentrations of organic acid compounds and flavones in S.scandens were 2.29-and 15.56-folds of those in S.scandens,respectively.Conclusion: The developed HPLC method is suitable for the fingerprint study for both of S.scandens and S.scandens.It is robust and producible enough to be used for the quality evaluation on S.scandens.

关键词(KeyWords)： 千里光;欧洲千里光;有机酸;黄酮;指纹图谱
Senecio scandens;S.vulgaris;organic acid compounds;flavones;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09052-013,2009ZX09052-021,2009ZX09052-024);; 国家自然科学基金项目(30701104);; 上海市国际合作基金项目(09540704700);; 中医药行业科研专项项目(200707009)

作者(Author): 杨雪晶;熊爱珍;杨莉;王长虹;王峥涛;
YANG Xuejing1,XIONG Aizhen2,YANG Li2,WANG Changhong2,WANG Zhengtao1,2,3* (1.Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China; 2.The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Shanghai Key Laboratory for Compound Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,Chin

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DOI:

参考文献(References)：

• [1]中国药典.一部[S].2010:32.
• [2]陈录新,马鸿雁,张勉,等.千里光化学成分研究[J].中国中药杂志,2006,31(22):1872.
• [3]史辑,张芳,马鸿雁,等.千里光化学成分研究[J].中国中药杂志,2007,32(15):1600.
• [4]张朝凤,陈录新,张勉,等.千里光化学成分研究[J].中国中药杂志,2008,33(18):2093.
• [5]Ortiz Cansado A,Crespo ValadésE,et al.Veno-occlusive liver dis-ease due to intake of Senecio vulgaris tea[J].GastroenterologiáyHepatologiá,1995,18(8):413.
• [6]Vilar J H,Garcia M,Cabrera P.Veno-occlusive liver disease in-duced by Senecio vulgaris toxicity[J].GastroenterologiáyHepatologiá,2000,23(6):285.
• [7]Li Y G,Zhang F,Wang Z T,et al.Identification and chemicalprofiling of monacolins in red yeast rice using high-performance liq-uid chromatography with photodiode array detector and mass spec-trometry[J].J Pharm Biomed Anal,2004,35:1101.
• [8]熊爱珍,杨莉,杨雪晶,等.RP-HPLC法测定千里光药材中金丝桃苷的含量[J].药物分析杂志,2010,30(5):936.