中国中药杂志

2015, v.40(23) 4645-4649

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白茅内生菌Chaetomium globosum的化学成分研究
Chemical constituents from endophyte Chaetomium globosum in Imperata cylindrical

申丽;朱力;魏中琦;李晓雯;李明;宋勇春;
SHEN Li;ZHU Li;WEI Zhong-qi;LI Xiao-wen;LI Ming;SONG Yong-chun;Medical College,Yangzhou University;Institute of Functional Biomolecules,Nanjing University;

摘要(Abstract):

采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等方法对白茅内生菌Chaetomium globosum固体发酵产物的化学成分进行分离纯化,获得9个化合物。利用高分辨质谱、核磁共振谱及文献比对的方法,将上述化合物分别鉴定为chaetoglobosin F(1),chaetoglobosin Fex(2),chaetoglobosin E(3),cytoglobosin A(4),penochalasin C(5),isochaetoglobosin D(6),N-benzoylphenylalaninyl-N-benzoylphenylalaninate(7),尿嘧啶(8),5-甲基尿嘧啶(9),其中化合物7为首次从毛壳菌中分离得到。采用MTT法测定化合物的体外细胞毒活性,结果显示,化合物1,3,4,5对人宫颈癌细胞株He La具有明显的抑制活性,IC50分别为99.43,23.77,97.92,86.25μmol·L~(-1),而阳性对照药顺铂的IC50为24.33μmol·L~(-1)。
Isolation and purification of chemical constituents from solid culture of endophyte Chaetomium globosum in Imperata cylindrical was performed through silica gel column chromatography,gel filtration over Sephadex LH-20 and preparative HPLC. Nine compounds were obtained and their structures were determined as chaetoglobosin F( 1),chaetoglobosin Fex( 2),chaetoglobosin E( 3),cytoglobosin A( 4),penochalasin C( 5),isochaetoglobosin D( 6),N-benzoylphenylalaninyl-N-benzoylphenylalaninate( 7),uracil( 8)and 5-methyluracil( 9),respectively,based on HR-MS and NMR data and comparison with literatures. Compound 7 was isolated from Chaetomium sp. for the first time. In vitro cytotoxicity of compounds was evaluated using MTT mothed and 1,3,4 and 5 showed inhibition activity to the human cervical carcinoma cell He La with IC50 values of 99. 43,23. 77,97. 92,86. 25 μmol·L~(-1),while positive control cisplatin had an IC50 value of 24. 33 μmol·L~(-1).

关键词(KeyWords): 白茅;内生菌;Chaetomium globosum;化学成分;细胞毒活性
Imperata cylindrical;endophyte;Chaetomium globosum;chemical constituent;cytotoxicity

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基金项目(Foundation): 国家自然科学基金项目(21372191,31370079);; 扬州市社会发展科技攻关项目(YZ2011097)

作者(Author): 申丽;朱力;魏中琦;李晓雯;李明;宋勇春;
SHEN Li;ZHU Li;WEI Zhong-qi;LI Xiao-wen;LI Ming;SONG Yong-chun;Medical College,Yangzhou University;Institute of Functional Biomolecules,Nanjing University;

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