刘训红;李俊松;张月婵;蔡宝昌;尹娣;
LIU Xunhong,LI Junsong,ZHANG Yuechan,CAI Baochang,YIN Di(Pharmacology College,Nanjing University of Chinese Medicine,Nangjing 210046,China)

• 1:南京中医药大学药学院

摘要(Abstract)：

目的:建立大黄饮片MEKC-DAD指纹图谱分析方法,并对大黄及其炮制品的指纹谱进行比较。方法:选择胶束电动毛细管电泳分离模式,以25 mmol.L-1硼砂-25 mmol.L-1SDS-10%乙腈(pH10.90)为运行缓冲液,分离电压12 kV,以大黄酸为参照物(IS),测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以11个共有峰为特征指纹信息的10批大黄地产药材MEKC-DAD指纹图谱;发现少数产地大黄药材的指纹图谱有一定差异,生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论:方法准确可靠,重复性好,可作为大黄饮片内在质量评价的依据。
Objective:To establish the analytical method for the fingerprint of Radix et Rhizoma Rhei by MEKC-DAD and compare the fingerprints of Radix et Rhizoma Rhei and its processed products.Method:Based on the mode of micellar electrokinetic chromatography,25 mmol·L-1 borax-25 mmol·L-1 SDS-10% acetonitrile was selected for the running buffer(pH 9.2).The separation voltage was 12 kV and the detection wavelength was set at 254 nm.Rhein was used as a reference standard,the chromatographic fingerprint was determined via the data analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples.Result: MEKC-DAD fingerprints with 11 common peaks of 10 batches of Radix et Rhizoma Rhei from the place of the genuine were established preliminarily.It was discovered that a small number of samples differed from others.Regarding to the fingerprints of Radix et Rhizoma Rhei and its processed products,there were obvious differences in the relative areas of common peaks.Conclusion: The method is reliable,accurate and can be used for quality control of Radix et Rhizoma Rhei.

关键词(KeyWords)： 大黄;胶束电动毛细管色谱二极管阵列检测法;指纹图谱
Radix et Rhizoma Rhei;MEKC-DAD;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 江苏省中药炮制重点实验室开放式课题(ZYPZ007)

作者(Author): 刘训红;李俊松;张月婵;蔡宝昌;尹娣;
LIU Xunhong,LI Junsong,ZHANG Yuechan,CAI Baochang,YIN Di(Pharmacology College,Nanjing University of Chinese Medicine,Nangjing 210046,China)

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DOI:

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