中国中药杂志

2009, v.34(23) 3034-3038

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

大黄饮片MEKC-DAD指纹图谱的研究
MEKC-DAD fingerprint of Radix et Rhizoma Rhei

刘训红;李俊松;张月婵;蔡宝昌;尹娣;
LIU Xunhong,LI Junsong,ZHANG Yuechan,CAI Baochang,YIN Di(Pharmacology College,Nanjing University of Chinese Medicine,Nangjing 210046,China)

摘要(Abstract):

目的:建立大黄饮片MEKC-DAD指纹图谱分析方法,并对大黄及其炮制品的指纹谱进行比较。方法:选择胶束电动毛细管电泳分离模式,以25 mmol.L-1硼砂-25 mmol.L-1SDS-10%乙腈(pH10.90)为运行缓冲液,分离电压12 kV,以大黄酸为参照物(IS),测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以11个共有峰为特征指纹信息的10批大黄地产药材MEKC-DAD指纹图谱;发现少数产地大黄药材的指纹图谱有一定差异,生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论:方法准确可靠,重复性好,可作为大黄饮片内在质量评价的依据。
Objective:To establish the analytical method for the fingerprint of Radix et Rhizoma Rhei by MEKC-DAD and compare the fingerprints of Radix et Rhizoma Rhei and its processed products.Method:Based on the mode of micellar electrokinetic chromatography,25 mmol·L-1 borax-25 mmol·L-1 SDS-10% acetonitrile was selected for the running buffer(pH 9.2).The separation voltage was 12 kV and the detection wavelength was set at 254 nm.Rhein was used as a reference standard,the chromatographic fingerprint was determined via the data analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples.Result: MEKC-DAD fingerprints with 11 common peaks of 10 batches of Radix et Rhizoma Rhei from the place of the genuine were established preliminarily.It was discovered that a small number of samples differed from others.Regarding to the fingerprints of Radix et Rhizoma Rhei and its processed products,there were obvious differences in the relative areas of common peaks.Conclusion: The method is reliable,accurate and can be used for quality control of Radix et Rhizoma Rhei.

关键词(KeyWords): 大黄;胶束电动毛细管色谱二极管阵列检测法;指纹图谱
Radix et Rhizoma Rhei;MEKC-DAD;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 江苏省中药炮制重点实验室开放式课题(ZYPZ007)

作者(Author): 刘训红;李俊松;张月婵;蔡宝昌;尹娣;
LIU Xunhong,LI Junsong,ZHANG Yuechan,CAI Baochang,YIN Di(Pharmacology College,Nanjing University of Chinese Medicine,Nangjing 210046,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享